Human IL-31 RA Biotinylated Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-31 RA in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) IL-31 RA is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL-31 RA
    Ala20-Ser516
    Accession # Q8NI17
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human IL‑31 RA (Catalog # 2769-IL)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-31 RA

The interleukin-31 receptor A subunit (IL-31 RA), also known as gp130-Like Monocyte Receptor (GLM-R or GPL), is a ~100 kDa type I transmembrane glycoprotein that is classified as being a type I cytokine receptor (1, 2). A heterodimeric complex of IL-31 RA and the oncostatin M receptor (OSM-R) functions as the signaling receptor for IL-31 (3). Both subunits are inducibly expressed throughout the myelomonocytic lineage and are upregulated by interferon-gamma and bacterial lipopolysaccharides (1‑3). IL-31 RA is also expressed on keratinocytes, dorsal root ganglia neurons, and variably on lung epithelial cells (3‑6). The 732 amino acid (aa) IL-31 RA contains a 19 aa signal sequence, a 500 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 192 aa cytoplasmic domain. The ECD shares 60%, 58%, 73% and 70% aa identity with mouse, rat, canine and bovine IL-31 RA ECD, respectively. Human IL-31 receptors do not respond to mouse IL-31 (7). The ECD contains five fibronectin type III domains; the first two contain four conserved cysteine residues and a WSXWS motif common to type I cytokine receptors (2). Twelve alternately spliced human IL-31 RA isoforms are known and range in size from 356‑745 amino acids. A long (745 aa) and a short (560 aa) transmembrane form are the predominant forms, and many cell lines express both forms (8). The long form, like the 732 aa form, signals by recruiting STAT3, 5 or 1, while the short form does not recruit STATs and inhibits IL-31 signaling. The ratio of these forms and their co-expression with OSM-R determines a cell’s response to IL‑31 (8). In both humans and transgenic mice, IL-31 from skin-homing Th2 cells may contribute to the pruritis (itching) associated with nonatopic dermatitis, especially in infected skin (3, 9, 10).

  • References:
    1. Ghilardi, N. et al. (2002) J. Biol. Chem. 277:16831.
    2. Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.
    3. Dillon, S. R. et al. (2004) Nat. Immunol. 5:752.
    4. Chattopadhyay, S. et al. (2007) J. Biol. Chem. 282:3014.
    5. Perrigoue, J. G. et al. (2007) J. Exp. Med. 204:481.
    6. Bando, T. et al. (2006) Neuroscience 142:1263.
    7. Broxmeyer, H. E. et al. (2007) Exp. Hematol. 35:78.
    8. Diveu, C. et al. (2004) Eur. Cytokine. Netw. 15:291.
    9. Bilsborough, J. et al. (2006) J. Allergy Clin. Immunol. 117:418.
    10. Sonkoly, E. et al. (2006) J. Allergy Clin. Immunol. 117:411.
  • Long Name:
    Interleukin 31 Receptor A
  • Entrez Gene IDs:
    133396 (Human); 218624 (Mouse)
  • Alternate Names:
    class I cytokine receptor; CRL3; CRL3Glmr; Cytokine receptor-like 3; GLMR; GLM-R; GLM-RMGC125346; GP130 like receptor; Gp130-like monocyte receptor; Gp130-like receptor; hGLM-R; IL-31 RA; IL-31 receptor subunit alpha; IL-31R subunit alpha; IL31RA; IL-31RA; IL-31RAGPLCRL; IL-31R-alpha; interleukin 31 receptor A; interleukin-31 receptor subunit alpha; PRO21384; soluble type I cytokine receptor CRL3; zcytoR17
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Interleukin (IL)-31 induces pro-inflammatory cytokines in human monocytes and macrophages following stimulation with staphylococcal exotoxins.
    Authors: Kasraie S, Niebuhr M, Werfel T
    Allergy, 2010;65(6):712-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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