Human IL-32 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
IC30402A
Detection of IL‑32 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (3)
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Human IL-32 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-32 alpha and other IL-32 isoforms in direct ELISAs. In direct ELISAs, 30% cross-reactivity with recombinant human (rh) IL-32 beta and 38% cross-reactivity with rhIL-32 gamma is detected.
Source
Monoclonal Rat IgG2A Clone # 373821
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-32 alpha
Met1-Asn131
Accession # P24001
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL-32 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of IL‑32 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA and calcium ionomycin were stained with Rat Anti-Human IL-32 APC-conjugated Monoclonal Antibody (Catalog # IC30402A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-32

IL-32 alpha is one of at least nine potential splice variants of the proinflammatory cytokine IL-32 (previously called NK4), which when full-length is 31 kDa in size. The predicted molecular weight of IL-32 alpha is approximately 15 kDa and it represents amino acids (aa) 1-18, 65-153, and 211-234 of the full-length (234 aa) form. Since the signal sequence of the ORF is aa 1-30, this isofom is like found intracellularly. IL-32 is induced by mitogens in peripheral lymphocytes, by IFN‑ gamma in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn Induces cytokine expression. No ortholog has been found in mouse.

Long Name
Interleukin 32
Entrez Gene IDs
9235 (Human)
Alternate Names
IL32; IL-32; IL-32alpha; IL-32beta; interleukin 32; interleukin-32 theta; interleukin-32; natural killer cell transcript 4; Natural killer cells protein 4; NK4; NK4IL-32delta; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; TAIFIL-32gamma; Tumor necrosis factor alpha-inducing factor

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Citations for Human IL-32 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Defective expansion and function of memory like natural killer cells in HIV+ individuals with latent tuberculosis infection
    Authors: KP Devalraju, VSK Neela, SS Krovvidi, R Vankayalap, VL Valluri
    PLoS ONE, 2021-09-13;16(9):e0257185.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Role of IL-32 in HIV Reactivation and its Link to HIV-HSV Coinfection
    Authors: Pedro M M Mesquita
    J. Infect. Dis, 2017-02-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure.
    Authors: Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D
    PLoS ONE, 2015-08-04;10(8):e0134568.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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