Human IL-37/IL-1F7 Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human IL-37/IL-1F7 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 899826
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IL-37/IL-1F7
    Accession # Q9NZH6
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑37/IL-1F7 in Human PBMCs Monocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) monocytes treated with 250 ng/mL LPS and 3 μM monensin overnight were stained with Mouse Anti-Human IL‑37/IL-1F7 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC19751G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-37/IL-1F7

Human interleukin 1 family member 7 (IL-1F7), also known as FIL-1Z, IL-1H4, and IL-1RP1, belongs to the IL-1 cytokine family, which currently has ten members. With the exception of IL-18 that maps to human chromosome 11, all other IL-1 family members map to the same cluster on human chromosome 2. Five alternatively spliced transcripts that arise through alternate exon usage have been described. These transcripts encode five different IL-1F7 isoforms (IL-1F7a through e also referred to as isofoms 1 through 5) that have distinct expression profiles. Polymorphism in the protein sequence of IL-1F7 isoforms also exists. Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-1F7 variants lack a typical signal peptide. The longest IL-1F7 transcript, referred to as IL-1F7b or IL-1F7 isoform 1, encodes a 218 amino acid (aa) residues proprotein containing a 45 aa propeptide, which is removed by caspase-1 to generate the 173 aa mature segment. Mature IL-1F7b and other IL-1F7 variants lack potential N-linked glycosylation sites. The secreted mature IL-17F7b was reported to exist as a nondisulfide linked homodimers in solution, IL-1F7 shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha, IL-1 beta and IL-1ra, respectively. Mouse IL-1F7 has not been reported, but human IL-1F7 is active on mouse cells. IL-1F7b binds to IL-18 R alpha with low affinity but does not exert any IL-18 agonistic or antagonistic effects. IL-1F7b also binds to the IL-18BP to enhance the antagonistic effects of IL-18BP. It has been proposed that IL-1F7b form a trimeric complex with IL-18BP and IL-18 R beta. This complex blocks IL-18 activity by sequestering the signal transducing subunit and preventing it from participating in IL-18 signaling (1‑8).

  • References:
    1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
    2. Kumar S. et al. (2000) J. Biol. Chem. 275:10308.
    3. Busfield S.J. et al. (2000) Genomics 66:213.
    4. Kumar S. et al. (2002) Cytokine 18:61.
    5. Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723.
    6. Gao, W. et al. (2003) J. Immunol. 170:1077.
    7. Pan, G. et al. (2001) Cytokine 13:1.
    8. Sims, J.E. et al. (2001) Trends Immunol. 22:533.
  • Long Name:
    Interleukin 37
  • Entrez Gene IDs:
    27178 (Human)
  • Alternate Names:
    FIL1 zeta; FIL1; FIL1(ZETA); FIL1ZFIL1 zeta; IL-1F7; IL-1F7IL-1 zeta; IL1H4IL1F7 (canonical product IL-1F7b); IL-1H4IL-1F7b (IL-1H4, IL-1H, IL-1RP1); IL-1RP1IL-1X protein; IL1RP1interleukin 1 family member 7; IL-1X; IL37; interleukin 1 family, member 7 (zeta); interleukin 1, zeta; interleukin-1 family member 7; Interleukin-1 homolog 4; interleukin-1 superfamily z; Interleukin-1 zeta; Interleukin-1-related protein
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC0041G  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human IL-37/IL-1F7 Alexa Fluor® 488-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee

Associated Pathways


Customer Information