Human IL-5 High Sensitivity Magnetic Luminex® Performance Assay

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Quantist software graph of signal intensity versus concentration for IL-5 in the Human High Sensitivity Cytokine A Luminex Panel
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Human IL-5 High Sensitivity Magnetic Luminex® Performance Assay Summary

Panel Luminex Performance Human High Sensitivity Cytokine Panel A
Luminex Performance Human High Sensitivity Cytokine Panel B
Assay Type Magnetic bead-based multiplex assay for the Luminex® platform
Format 1 x 96-well microplate and Magnetic antibody-coated beads
Species Human
Analytes Detected Please see analyte list in assay customization tool below.
Performance Validation Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

Make your selections below to configure your assay

Please choose one of the panels to display the corresponding analytes.

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Assays

Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If you run into bead region incompatibilities, send your Luminex code to our team and find out more.

How would you like these analytes mixed?

Premixed
User Mixed
Please select at least one mixing option to see the kits.

Would you like to split based on dilution?

Do not split
Split based on serum
Split based on plasma
Split based on cell culture supernates

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.

Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

  • Simultaneously profile Human IL-5 with up to analytes of your choice in one sample
  • Magnetic format allows for easier wash steps
  • Undergoes similar validation testing as Quantikine® ELISA Kits
  • Precise and Specific
  • Requires a small sample volume (<50 µL)
  • Run your assay in just 3-5 hours
  • Mass-calibrated standards for consistent results with every new lot of material
  • Enough reagents for one 96-well plate
Base Kit Components
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Calibrator Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One flat-bottom 96-well Microplate
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Standard Value Card**

**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.

Bead Set Contents
  • User-mix antibody-coated Magnetic beads in individual vials
  • User-mixed cocktail of biotinylated detection antibody

Precision

Intra-assay Precision (precision within an assay) - Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (precision between assays) - Three samples of known concentration were tested in sixty separate assays to assess precision between assays.

  Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 60 60 60
Mean (pg/mL) 3.0 21 440 2.8 22 445
Standard deviation 0.2 0.7 32 0.4 2.0 53
CV (%) 6.7 3.3 7.3 14.3 9.1 11.9
 

Recovery

Samples containing and/or spiked with human IL-5 were evaluated for recovery.

Sample Type Average % Recovery Range
Cell culture supernates 104 94-113%
Serum 85 67-108%
EDTA plasma 84 62-105%
Heparin plasma 88 67-145%
 

Linearity

Samples containing and/or spiked with human IL-5 were serially diluted to evaluate assay linearity.

  Cell culture supernates* Serum EDTA plasma Heparin plasma
1:2 Average % of Expected 100 109 104 108
Range (%) 97-102 104-115 95-118 104-112
1:4 Average % of Expected 101 116 108 111
Range (%) 100-102 105-126 100-117 105-120
1:8 Average % of Expected 104 117 106 106
Range (%) 101-106 109-124 95-115 100-115


*Cell culture supernates are valid samples in Human HS Cytokine Base Kit B only.

Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.

Scientific Data

Quantist software graph of signal intensity versus concentration for IL-5 in the Human High Sensitivity Cytokine A Luminex Panel

Background: IL-5

IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.

Long Name
Interleukin 5
Entrez Gene IDs
3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
Alternate Names
BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I

Product Datasheets

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Product Specific Notices
Luminex® is a registered trademark of Luminex Corporation.

Citations for Human IL-5 High Sensitivity Magnetic Luminex® Performance Assay

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Patients with idiopathic recurrent miscarriage have abnormally high TGF�+ blood NK, NKT and T cells in the presence of abnormally low TGF� plasma levels
    Authors: L Zhu, M Aly, RJ Kuon, B Toth, H Wang, H Karakizlis, R Weimer, C Morath, E Ibrahim, N Ekpoom, G Opelz, V Daniel
    BMC Immunol., 2019-03-04;20(1):10.
    Species: Human
    Sample Types: Plasma
  2. The plasma interleukin-6 response to acute psychosocial stress in humans is detected by a magnetic multiplex assay: comparison to high-sensitivity ELISA
    Authors: AM Quinn, AR Williams, TI Sivilli, CL Raison, TWW Pace
    Stress, 2018-03-13;0(0):1-6.
    Species: Human
    Sample Types: Plasma
  3. Cytokines and Postoperative Delirium in Older Patients Undergoing Major Elective Surgery.
    Authors: Vasunilashorn S, Ngo L, Inouye S, Libermann T, Jones R, Alsop D, Guess J, Jastrzebski S, McElhaney J, Kuchel G, Marcantonio E
    J Gerontol A Biol Sci Med Sci, 2015-07-27;0(0):.
    Species: Human
    Sample Types: Plasma
  4. Clinical Scale Zinc Finger Nuclease-mediated Gene Editing of PD-1 in Tumor Infiltrating Lymphocytes for the Treatment of Metastatic Melanoma.
    Authors: Beane J, Lee G, Zheng Z, Mendel M, Abate-Daga D, Bharathan M, Black M, Gandhi N, Yu Z, Chandran S, Giedlin M, Ando D, Miller J, Paschon D, Guschin D, Rebar E, Reik A, Holmes M, Gregory P, Restifo N, Rosenberg S, Morgan R, Feldman S
    Mol Ther, 2015-05-05;23(8):1380-90.
    Species: Human
    Sample Types: Cell Culture Supernates

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