Human Integrin alpha 4/CD49d PE‑conjugated Antibody

Name: Human Integrin alpha 4/CD49d PE‑conjugated Antibody
Brand: R&D Systems
SKU: FAB1354P
Price: 319 USD
Availability: InStock

R&D Systems | Catalog # FAB1354P

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Citations (3)

Product Documents

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Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG₁ Clone # 7.2R

View all Integrin alpha 4/CD49d Primary Antibodies »

Product Specifications

Immunogen

DX-3 human melanoma cell line

Specificity

Detects the human Integrin alpha 4/CD49d subunit in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Integrin alpha 4/CD49d PE‑conjugated Antibody

Detection of Integrin alpha 4/CD49d in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin a4/CD49d PE-conjugated Monoclonal Antibody (Catalog # FAB1354P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin alpha 4/CD49d PE‑conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood lymphocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Integrin alpha 4/CD49d

The Integrin alpha 4 subunit, also known as CD49d and VLA-4 alpha subunit, forms heterodimers with Integrin beta 1 (CD29) or beta 7. Integrins alpha 4 beta 1 (VLA4) and alpha 4 beta 7 are both receptors for fibronectin and VCAM. Integrin alpha 4 beta 7 also binds the mucosal addressin cell adhesion molecule (MAdCAM-1). On the cell surface, VLA4 will participate in homotypic binding between endothelial cells and leukocytes. The extracellular domains of human and mouse Integrin alpha 4 share 85% aa sequence identity.