Human Integrin  alpha 4/CD49d PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1354P
Detection of Integrin  alpha 4/CD49d in Human Blood Lymphocytes by Flow Cytometry.
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Human Integrin  alpha 4/CD49d PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects the human Integrin alpha 4/CD49d subunit in flow cytometry.
Source
Monoclonal Mouse IgG1 Clone # 7.2R
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
DX-3 human melanoma cell line
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin a4/CD49d antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of Integrin alpha 4/CD49d in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin a4/CD49d PE-conjugated Monoclonal Antibody (Catalog # FAB1354P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Integrin alpha 4/CD49d

The Integrin  alpha 4 subunit, also known as CD49d and VLA-4 alpha subunit, forms heterodimers with Integrin beta 1 (CD29) or beta 7. Integrins alpha 4 beta 1 (VLA4) and alpha 4 beta 7 are both receptors for fibronectin and VCAM. Integrin alpha 4 beta 7 also binds the mucosal addressin cell adhesion molecule (MAdCAM-1). On the cell surface, VLA4 will participate in homotypic binding between endothelial cells and leukocytes. The extracellular domains of human and mouse Integrin alpha 4 share 85% aa sequence identity.

Entrez Gene IDs
3676 (Human); 16401 (Mouse); 102128433 (Cynomolgus Monkey)
Alternate Names
269C wild type; CD49 antigen-like family member D; CD49d antigen; CD49d; CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor; IA4; Integrin alpha 4; integrin alpha-4 subunit; integrin alpha-4; Integrin alpha-IV; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); ITGA4; MGC90518; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha; VLA-4

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