Human Integrin alpha 4/CD49d Biotinylated Antibody

Catalog #: BAM1354
1 Citation Datasheet / COA / SDS

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BAM1354

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Integrin alpha 4/CD49d in MOLT‑4 Human Cell Line.

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Human Integrin alpha 4/CD49d Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin alpha 4/CD49d.

Source

Monoclonal Mouse IgG₁ Clone # 7.2R

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

DX-3 human melanoma cell line

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

Human peripheral blood lymphocytes

Immunocytochemistry

8-25 µg/mL

Immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Integrin alpha 4/CD49d in MOLT‑4 Human Cell Line. Integrin alpha 4/CD49d was detected in immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human Integrin alpha 4/CD49d Biotinylated Monoclonal Antibody (Catalog # BAM1354) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 4/CD49d

The alpha 4 subunit, also known as CD49d and VLA-4 alpha subunit, forms heterodimers with Integrin beta 1 (CD29) or beta 7. Integrins alpha 4 beta 1 and alpha 4 beta 7 are receptors for fibronectin and VCAM. Integrin alpha 4 beta 1 also binds the mucosal addressin cell adhesion molecule (MAdCAM-1).

Entrez Gene IDs

3676 (Human); 16401 (Mouse); 102128433 (Cynomolgus Monkey)

Alternate Names

269C wild type; CD49 antigen-like family member D; CD49d antigen; CD49d; CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor; IA4; Integrin alpha 4; integrin alpha-4 subunit; integrin alpha-4; Integrin alpha-IV; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); ITGA4; MGC90518; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha; VLA-4

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human Integrin alpha 4/CD49d Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Multiple cellular antigen detection by ICP-MS.
Authors: Ornatsky O, Baranov VI, Bandura DR, Tanner SD, Dick J
J. Immunol. Methods, 2005-11-21;308(1):68-76.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry