Human Integrin alpha 4/CD49d Biotinylated Antibody

Name: Human Integrin alpha 4/CD49d Biotinylated Antibody
Brand: R&D Systems
SKU: BAM1354
Price: 459 USD
Availability: InStock

R&D Systems | Catalog # BAM1354

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry

Cited:

Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ Clone # 7.2R

View all Integrin alpha 4/CD49d Primary Antibodies »

Product Specifications

Immunogen

DX-3 human melanoma cell line

Specificity

Detects human Integrin alpha 4/CD49d.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Integrin alpha 4/CD49d Biotinylated Antibody

Integrin alpha 4/CD49d in MOLT‑4 Human Cell Line.

Integrin alpha 4/CD49d was detected in immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human Integrin alpha 4/CD49d Biotinylated Monoclonal Antibody (Catalog # BAM1354) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Integrin alpha 4/CD49d Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human peripheral blood lymphocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 4/CD49d

The alpha 4 subunit, also known as CD49d and VLA-4 alpha subunit, forms heterodimers with Integrin beta 1 (CD29) or beta 7. Integrins alpha 4 beta 1 and alpha 4 beta 7 are receptors for fibronectin and VCAM. Integrin alpha 4 beta 1 also binds the mucosal addressin cell adhesion molecule (MAdCAM-1).