Human Integrin  alpha 4/CD49d Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM1354
Integrin  alpha 4/CD49d in MOLT‑4 Human Cell Line.
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Citations (1)
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Human Integrin  alpha 4/CD49d Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha 4/CD49d.
Source
Monoclonal Mouse IgG1 Clone # 7.2R
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
DX-3 human melanoma cell line
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood lymphocytes
Immunocytochemistry
8-25 µg/mL
Immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry View Larger

Integrin  alpha 4/CD49d in MOLT‑4 Human Cell Line. Integrin  alpha 4/CD49d was detected in immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human Integrin  alpha 4/CD49d Biotinylated Monoclonal Antibody (Catalog # BAM1354) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 4/CD49d

The alpha 4 subunit, also known as CD49d and VLA-4 alpha subunit, forms heterodimers with Integrin beta 1 (CD29) or beta 7. Integrins alpha 4 beta 1 and alpha 4 beta 7 are receptors for fibronectin and VCAM. Integrin alpha 4 beta 1 also binds the mucosal addressin cell adhesion molecule (MAdCAM-1).

Entrez Gene IDs
3676 (Human); 16401 (Mouse); 102128433 (Cynomolgus Monkey)
Alternate Names
269C wild type; CD49 antigen-like family member D; CD49d antigen; CD49d; CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor; IA4; Integrin alpha 4; integrin alpha-4 subunit; integrin alpha-4; Integrin alpha-IV; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); ITGA4; MGC90518; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha; VLA-4

Product Datasheets

Citation for Human Integrin  alpha 4/CD49d Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Multiple cellular antigen detection by ICP-MS.
    Authors: Ornatsky O, Baranov VI, Bandura DR, Tanner SD, Dick J
    J. Immunol. Methods, 2005;308(1):68-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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