Human Integrin  alpha 4/CD49d Antibody

Catalog # Availability Size / Price Qty
MAB1354-SP
MAB1354-100
MAB1354-500
Detection of Integrin  alpha 4/CD49d in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (6)
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Human Integrin  alpha 4/CD49d Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha 4/CD49d. Recognizes the human Integrin alpha 4/CD49d subunit.
Source
Monoclonal Mouse IgG1 Clone # 7.2R
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
DX-3 human melanoma cell line
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Integrin a4/CD49d antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of Integrin alpha 4/CD49d in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin a4/CD49d Monoclonal Antibody (Catalog # MAB1354, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry Integrin a4/CD49d antibody in U937 Human Cell Line by Immunocytochemistry (ICC). View Larger

Integrin alpha 4/CD49d in U937 Human Cell Line. Integrin a4/ CD49d was detected in immersion fixed U937 human histiocytic lymphoma cell line using 10 µg/mL Mouse Anti-Human Integrin a4/CD49d Monoclonal Antibody (Catalog # MAB1354) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 4/CD49d

The alpha 4 subunit, also known as CD49d and VLA-4 alpha subunit, forms heterodimers with Integrin beta 1 (CD29) or beta 7. Integrins alpha 4 beta 1 and alpha 4 beta 7 are receptors for fibronectin and VCAM. Integrin alpha 4 beta 1 also binds the mucosal addressin cell adhesion molecule (MAdCAM-1).

Entrez Gene IDs
3676 (Human); 16401 (Mouse); 102128433 (Cynomolgus Monkey)
Alternate Names
269C wild type; CD49 antigen-like family member D; CD49d antigen; CD49d; CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor; IA4; Integrin alpha 4; integrin alpha-4 subunit; integrin alpha-4; Integrin alpha-IV; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); ITGA4; MGC90518; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha; VLA-4

Product Datasheets

Citations for Human Integrin  alpha 4/CD49d Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Targeting VLA4 integrin and CXCR2 mobilizes serially repopulating hematopoietic stem cells
    Authors: D Karpova, MP Rettig, J Ritchey, D Cancilla, S Christ, L Gehrs, E Chendamara, MO Evbuomwan, M Holt, J Zhang, G Abou-Ezzi, H Celik, E Wiercinska, W Yang, F Gao, LG Eissenberg, RF Heier, SD Arnett, MJ Meyers, MJ Prinsen, DW Griggs, A Trumpp, PG Ruminski, DM Morrow, HB Bonig, DC Link, JF DiPersio
    J. Clin. Invest., 2019;129(7):2745-2759.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  2. C6-ceramide nanoliposome suppresses tumor metastasis by eliciting PI3K and PKCzeta tumor-suppressive activities and regulating integrin affinity modulation.
    Authors: Zhang P, Fu C, Hu Y, Dong C, Song Y, Song E
    Sci Rep, 2015;5(0):9275.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.
    Authors: Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE
    Arthritis Rheum., 2007;56(6):1817-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
    Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
    Dig. Dis. Sci., 2007;52(10):2775-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
    Authors: Carman CV, Springer TA
    J. Cell Biol., 2004;167(2):377-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
    Authors: Carman CV, Springer TA
    J. Cell Biol., 2004;167(2):377-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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