Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # P5H9
View all Integrin alpha V beta 5 Primary Antibodies »

Product Specifications

Immunogen

HT1080 human fibrosarcoma cell line

Specificity

Detects human Integrin alpha V beta 5. Recognizes the human Integrin alpha V beta 5 heterodimer and does not recognize the alpha V subunit in association with any other beta subunits.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Integrin  alpha V beta 5 APC‑conjugated Antibody

Detection of Integrin  alpha V beta 5 in HT1080 cells by Flow Cytometry

HT1080 cells were stained with Mouse Anti-Human Integrin  alpha V beta 5 APC‑conjugated Monoclonal Antibody (Catalog # FAB2528A) isotype control antibody (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Detection of Integrin aV beta 5 antibody in MCF‑7 Human Cell Line antibody by Flow Cytometry.

Detection of Integrin alpha V beta 5 in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Integrin aV beta 5 APC-conjugated Monoclonal Antibody (Catalog # FAB2528A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin  alpha V beta 5 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HT1080 human fibrosarcoma cell line and MCF‑7 human breast cancer cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha V beta 5

Integrins are heterodimeric receptors comprised of an alpha and a beta subunit. Integrin alpha V (CD51) associates with several different beta subunits, but Integrin beta 5 associates exclusively with the alpha V subunit. Integrin alpha V beta 5, also known as Integrin alpha V beta S and Integrin alpha V beta 3B, is a transmembrane heterodimeric protein that functions as a receptor for Vitronectin. It is expressed on hepatoma cells, fibroblasts and carcinoma cells.

Alternate Names

CD51, integrin subunit alpha V, ITGAV, MSK8, VNRA, VTNR

Entrez Gene IDs

3685 (Human)

Gene Symbol

ITGAV

Additional Integrin alpha V beta 5 Products

Product Documents for Human Integrin  alpha V beta 5 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Integrin  alpha V beta 5 APC‑conjugated Antibody

For research use only

Citations for Human Integrin  alpha V beta 5 APC‑conjugated Antibody

Customer Reviews for Human Integrin  alpha V beta 5 APC‑conjugated Antibody

There are currently no reviews for this product. Be the first to review Human Integrin  alpha V beta 5 APC‑conjugated Antibody and earn rewards!

Have you used Human Integrin  alpha V beta 5 APC‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies