hh Human Integrin  alpha V beta 5 PE-conjugated Antibody FAB2528P: R&D Systems

Human Integrin  alpha V beta 5 PE-conjugated Antibody

(1 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Integrin alpha V beta 5. Recognizes the human Integrin alpha V beta 5 heterodimer and does not recognize the alpha V subunit in association with any other beta subunits.
  • Source
    Monoclonal Mouse IgG1 Clone # P5H9
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    HT1080 human fibrosarcoma cell line
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Integrin  alpha V beta 5 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human Integrin  alpha V beta 5 PE‑conjugated Monoclonal Antibody (Catalog # FAB2528P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha V beta 5
Integrins are heterodimeric receptors comprised of an alpha and a beta subunit. Integrin alpha V (CD51) associates with several different beta subunits, but Integrin beta 5 associates exclusively with the alpha V subunit. Integrin alpha V beta 5, also known as Integrin alpha V beta S and Integrin alpha V beta 3B, is a transmembrane heterodimeric protein that functions as a receptor for Vitronectin. It is expressed on hepatoma cells, fibroblasts and carcinoma cells.
  • Entrez Gene IDs:
    3685 (Human)
  • Alternate Names:
    Integrin alpha V beta 5
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Development of novel AAV serotype 6 based vectors with selective tropism for human cancer cells.
    Authors: Sayroo R, Nolasco D, Yin Z, Colon-Cortes Y, Pandya M, Ling C, Aslanidi G
    Gene Ther, 2016;23(1):18-25.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 38  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Flow Cytometry Human Integrin  alpha V beta 5 PE-conjugated Antibody FAB2528P
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Very Good
 Flow Human Anonymous 08/01/2016
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Flow Cytometry Human Integrin  alpha V beta 5 PE-conjugated Antibody FAB2528P
Flow Cytometry: Human Integrin  alpha V beta 5 PE-conjugated Antibody [FAB2528P]

Summary

ApplicationFlow Cytometry
Sample TestedBone marrow cells
SpeciesHuman

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