Human Integrin  alpha V beta 5 Antibody

Catalog # Availability Size / Price Qty
MAB2528
MAB2528-SP
Human Integrin  alpha V beta 5 Antibody in Data
1 Image
Product Details
Citations (11)
FAQs
Supplemental Products
Reviews (1)

Human Integrin  alpha V beta 5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha V beta 5. Recognizes the human Integrin alpha V beta 5 heterodimer and does not recognize the alpha V subunit in association with any other beta subunits.
Source
Monoclonal Mouse IgG1 Clone # P5H9
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HT1080 human fibrosarcoma cell line
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
MCF‑7 human breast cancer cell line
Immunoprecipitation
Wayner, E.A. et al. (1991) J. Cell Biol. 113:919.
 
Adhesion Blockade
Wayner, E.A. et al. (1991) J. Cell Biol. 113:919.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Integrin  alpha V beta 5 in HT1080 Human Cell Line. View Larger

Integrin alpha V beta 5 in HT1080 Human Cell Line. Integrin aV beta 5 was detected in immersion fixed HT1080 human fibrosarcoma cell line using Human Integrin aV beta 5 Monoclonal Antibody (Catalog # MAB2528) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha V beta 5

Integrins are heterodimeric receptors comprised of an alpha and a beta subunit. Integrin alpha V (CD51) associates with several different beta subunits, but Integrin beta 5 associates exclusively with the alpha V subunit. Integrin alpha V beta 5, also known as Integrin alpha V beta S and Integrin alpha V beta 3B, is a transmembrane heterodimeric protein that functions as a receptor for Vitronectin. It is expressed on hepatoma cells, fibroblasts and carcinoma cells.

Entrez Gene IDs
3685 (Human)
Alternate Names
CD51; Integrin alpha V beta 5; integrin subunit alpha V; ITGAV; MSK8; VNRA; VTNR

Product Datasheets

Citations for Human Integrin  alpha V beta 5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. GSH-sensitive Pt(IV) prodrug-loaded phase-transitional nanoparticles with a hybrid lipid-polymer shell for precise theranostics against ovarian cancer
    Authors: H Huang, Y Dong, Y Zhang, D Ru, Z Wu, J Zhang, M Shen, Y Duan, Y Sun
    Theranostics, 2019;9(4):1047-1065.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. The post-abscission midbody is an intracellular signaling organelle that regulates cell proliferation
    Authors: E Peterman, P Gibieža, J Schafer, VA Skeberdis, A Kaupinis, M Valius, X Heiligenst, I Hurbain, G Raposo, R Prekeris
    Nat Commun, 2019;10(1):3181.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. The oncolytic adenovirus VCN-01 promotes anti-tumor effect in primitive neuroectodermal tumor models
    Authors: M Garcia-Mou, N Martinez-V, M Gonzalez-H, L Marrodán, M Cascallo, R Alemany, A Patiño-Gar, MM Alonso
    Sci Rep, 2019;9(1):14368.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Osteopontin mediates glioblastoma-associated macrophage infiltration and is a therapeutic target
    Authors: J Wei, A Marisetty, B Schrand, K Gabrusiewi, Y Hashimoto, M Ott, Z Grami, LY Kong, X Ling, HG Caruso, S Zhou, YA Wang, GN Fuller, JT Huse, E Gilboa, N Kang, X Huang, R Verhaak, S Li, AB Heimberger
    J. Clin. Invest., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. cRGD peptide installation on cisplatin-loaded nanomedicines enhances efficacy against locally advanced head and neck squamous cell carcinoma bearing cancer stem-like cells
    Authors: K Miyano, H Cabral, Y Miura, Y Matsumoto, Y Mochida, H Kinoh, K Iwata, O Nagano, H Saya, N Nishiyama, K Kataoka, T Yamasoba
    J Control Release, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Arg-Gly-Asp (RGD)-Modified E1A/E1B Double Mutant Adenovirus Enhances Antitumor Activity in Prostate Cancer Cells In Vitro and in Mice.
    Authors: Shen Y, Yang F, Wang H, Cai Z, Xu Y, Zhao A, Su Y, Zhang G, Zhu S
    PLoS ONE, 2016;11(1):e0147173.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Direct contact with perivascular tumor cells enhances integrin �v?3 signaling and migration of endothelial cells
    Authors: ME Burgett, JD Lathia, P Roth, AS Nowacki, DS Galileo, E Pugacheva, P Huang, A Vasanji, M Li, T Byzova, T Mikkelsen, S Bao, JN Rich, M Weller, CL Gladson
    Oncotarget, 2016;7(28):43852-43867.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Phagocytosis of apoptotic or necrotic cells differentially regulates the transcriptional expression of IL-12 family members in dendritic cells.
    Authors: Dixon K, O'Flynn J, van der Kooij S, van Kooten C
    J Leukoc Biol, 2014;96(2):313-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Involvement of angiopoietin-like 4 in matrix remodeling during chondrogenic differentiation of mesenchymal stem cells.
    Authors: Mathieu M, Iampietro M, Chuchana P, Guerit D, Djouad F, Noel D, Jorgensen C
    J Biol Chem, 2014;289(12):8402-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  10. Degradation of internalized alphaVbeta5 integrin is controlled by uPAR bound uPA: effect on beta1 integrin activity and alpha-SMA stress fiber assembly.
    Authors: Wang L, Pedroja BS, Meyers EE, Garcia AL, Twining SS, Bernstein AM
    PLoS ONE, 2012;7(3):e33915.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
    Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
    Immunol. Lett., 2011;134(2):167-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Integrin  alpha V beta 5 Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human Integrin  alpha V beta 5 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human Integrin  alpha V beta 5 Antibody
By Anonymous on 01/10/2017
Application: Flow Sample Tested: Mesenchymal stem cells Species: Human