Human Integrin  alpha V beta 5 Antibody

(6 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Integrin alpha V beta 5. Recognizes the human Integrin alpha V beta 5 heterodimer and does not recognize the alpha V subunit in association with any other beta subunits.
  • Source
    Monoclonal Mouse IgG1 Clone # P5H9
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    HT1080 human fibrosarcoma cell line
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    MCF‑7 human breast cancer cell line
  • Immunoprecipitation
    Wayner, E.A. et al. (1991) J. Cell Biol. 113:919.
  • Adhesion Blockade
    Wayner, E.A. et al. (1991) J. Cell Biol. 113:919.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Integrin  alpha V beta 5 in HT1080 Human Cell Line. Integrin  alpha V beta 5 was detected in immersion fixed HT1080 human fibrosarcoma cell line using Human Integrin  alpha V beta 5 Monoclonal Antibody (Catalog # MAB2528) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha V beta 5
Integrins are heterodimeric receptors comprised of an alpha and a beta subunit. Integrin alpha V (CD51) associates with several different beta subunits, but Integrin beta 5 associates exclusively with the alpha V subunit. Integrin alpha V beta 5, also known as Integrin alpha V beta S and Integrin alpha V beta 3B, is a transmembrane heterodimeric protein that functions as a receptor for Vitronectin. It is expressed on hepatoma cells, fibroblasts and carcinoma cells.
  • Entrez Gene IDs:
    3685 (Human)
  • Alternate Names:
    Integrin alpha V beta 5
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Direct contact with perivascular tumor cells enhances integrin �v?3 signaling and migration of endothelial cells
    Authors: ME Burgett, JD Lathia, P Roth, AS Nowacki, DS Galileo, E Pugacheva, P Huang, A Vasanji, M Li, T Byzova, T Mikkelsen, S Bao, JN Rich, M Weller, CL Gladson
    Oncotarget, 2016;7(28):43852-43867.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Arg-Gly-Asp (RGD)-Modified E1A/E1B Double Mutant Adenovirus Enhances Antitumor Activity in Prostate Cancer Cells In Vitro and in Mice.
    Authors: Shen Y, Yang F, Wang H, Cai Z, Xu Y, Zhao A, Su Y, Zhang G, Zhu S
    PLoS ONE, 2016;11(1):e0147173.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Involvement of angiopoietin-like 4 in matrix remodeling during chondrogenic differentiation of mesenchymal stem cells.
    Authors: Mathieu M, Iampietro M, Chuchana P, Guerit D, Djouad F, Noel D, Jorgensen C
    J Biol Chem, 2014;289(12):8402-12.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC
  4. Phagocytosis of apoptotic or necrotic cells differentially regulates the transcriptional expression of IL-12 family members in dendritic cells.
    Authors: Dixon K, O'Flynn J, van der Kooij S, van Kooten C
    J Leukoc Biol, 2014;96(2):313-24.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Degradation of internalized alphaVbeta5 integrin is controlled by uPAR bound uPA: effect on beta1 integrin activity and alpha-SMA stress fiber assembly.
    Authors: Wang L, Pedroja BS, Meyers EE, Garcia AL, Twining SS, Bernstein AM
    PLoS ONE, 2012;7(3):e33915.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
    Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
    Immunol. Lett., 2011;134(2):167-73.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Flow Cytometry Human Integrin  alpha V beta 5 Antibody MAB2528
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 Flow Human Anonymous 01/10/2017
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Flow Cytometry Human Integrin  alpha V beta 5 Antibody MAB2528
Flow Cytometry: Human Integrin  alpha V beta 5 Antibody [MAB2528]

Summary

ApplicationFlow Cytometry
Sample TestedMesenchymal stem cells
SpeciesHuman

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