Detects human Integrin beta 3/CD61 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Integrin beta 1, beta 2, recombinant mouse Integrin beta 4, or beta 6 is observed.
Monoclonal Mouse IgG2A Clone # 256809
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 3/CD61 Gly27-Asp718 Accession # P05106
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Integrin beta 3/CD61 in Human Peripheral Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Integrin beta 3/CD61 APC‑conjugated Monoclonal Antibody (Catalog # FAB2266A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin beta 3/CD61
Integrin beta 3, also called CD61, associates with the Integrin alpha IIb or alpha V
subunits to form heterodimeric receptors for collagens, fibronectin,
vitronectin, fibrinogen, Von willebrand factor or thrombospondin.
alpha IIb/ beta 3, also known as GPIIb IIIa (Glycoprotein IIb-IIIa), is expressed
on platelets. alpha V/ beta 3, also known as vitronectin receptor, is expressed
on endothelial cells, some B cells, platelets and monocytes.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.