Detects human Integrin beta 3/CD61 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Integrin beta 1, beta 2, recombinant mouse Integrin beta 4, or beta 6 is observed.
Monoclonal Mouse IgG2A Clone # 256809
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 3/CD61 Gly27-Asp718 Accession # P05106
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Integrin beta 3/CD61 in Human Blood Monocytes by Flow Cytometry.
Human peripheral blood monocytes were stained with Mouse Anti-Human Integrin beta 3/CD61 PE‑conjugated Monoclonal Antibody (Catalog # FAB2266P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin beta 3/CD61
Integrin beta 3, also called CD61, associates with the Integrin alpha IIb or alpha V subunits to form heterodimeric receptors for Collagens, Fibronectin, Vitronectin, Fibrinogen, Von Willebrand factor or Thrombospondin. alpha IIb/ beta 3, also known as GPIIb IIIa (Glycoprotein IIb-IIIa), is expressed on platelets. alpha V/ beta 3, also known as Vitronectin Receptor, is expressed on endothelial cells, some B cells, platelets and monocytes.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.