Detects human Integrin beta 4/CD104 in Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta 1, beta 2, beta 3, beta 5, beta 6, beta 8, or recombinant mouse Integrin beta 4 is observed.
Monoclonal Mouse IgG2B Clone # 422325
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 4/CD104 Asn28-Ser710 Accession # P16144
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 488
5 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Integrin beta 4/CD104 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Integrin beta 4/CD104 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB4060G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin beta 4/CD104
Integrin beta 4 (also GP150 and CD104) is a 150-200 kDa member of the integrin beta family. It forms noncovalent heterodimers with integrin alpha 6 and participates in the formation of epithelial hemidesmosomes. Human integrin beta 4 is a type I transmembrane glycoptotein that is 1795 amino acids (aa) in length. It contains a 683 aa extracellular domain (ECD) (aa 28-710) and a large 1089 aa cytoplasmic region. Two ECD alternate splice forms may exist. One shows an 84 aa substitution for the 84 aa between aa 621-704. A second shows a 144 aa substitution for the first 704 amino acids. Human beta 4 ECD shares 88% aa sequence identity with mouse beta 4 ECD.
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