Human Integrin beta 4/CD104 APC-conjugated Antibody
Human Integrin beta 4/CD104 APC-conjugated Antibody Summary
Accession # P16144
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Integrin beta 4/CD104 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Integrin beta 4/CD104 APC-conjugated Monoclonal Antibody (Catalog # FAB4060A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Background: Integrin beta 4/CD104
Integrin beta 4 (also GP150 and CD104) is a 150‑200 kDa member of the integrin beta family of proteins. It forms noncovalent heterodimers with integrin alpha 6 and participates in the formation of epithelial hemidesmosomes by binding Plexin 1A. Integrin alpha 6 beta 4 also binds Laminin. Human integrin beta 4 is a type I transmembrane glycoptotein that is 1795 amino acids (aa) in length. It contains a 683 aa extracellular domain (ECD) (aa 28‑710) and a large 1089 aa cytoplasmic region. One ECD alternate splice form is reported. One shows an 84 aa substitution for the 84 aa between aa 621‑704. A second shows a 144 aa substitution for the first 704 amino acids. Human beta 4 ECD shares 88% aa sequence identity with mouse beta 4 ECD.
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