Human Integrin  beta 4/CD104 Antibody

Catalog # Availability Size / Price Qty
MAB4060
MAB4060-SP

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Integrin  beta 4/CD104 in A431 Human Cell Line.
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Product Details
Citations (10)
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Reviews (2)

Human Integrin  beta 4/CD104 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin beta 4/CD104 in Western blots. In Western blots, no cross-reactivity with recombinant human Integrin  beta 1, beta 2, beta 3, beta 5, beta 6, beta 8, or recombinant mouse Integrin  beta 4 is observed.
Source
Monoclonal Mouse IgG2B Clone # 422325
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 4/CD104
Asn28-Ser710
Accession # P16144
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Integrin  beta 4/CD104 under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
A431 human epithelial carcinoma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Integrin  beta 4/CD104 antibody in A431 Human Cell Line by Immunocytochemistry (ICC). View Larger

Integrin beta 4/CD104 in A431 Human Cell Line. Integrin beta 4/CD104 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human Integrin beta 4/CD104 Monoclonal Antibody (Catalog # MAB4060) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 4/CD104

Integrin beta 4 (also GP150 and CD104) is a 150‑200 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha 6 and participates in the formation of epithelial hemidesmosomes. Human Integrin beta 4 is a type I transmembrane glycoprotein that is 1795 amino acids (aa) in length. It contains a 683 aa extracellular domain (ECD) (aa 28‑710) and a large 1089 aa cytoplasmic region. Two ECD alternative splice forms may exist. One shows an 84 aa substitution for the 84 aa between aa 621‑704. A second shows a 144 aa substitution for the first 704 amino acids. Human beta 4 ECD shares 88% aa sequence identity with mouse beta 4 ECD.

Entrez Gene IDs
3691 (Human); 192897 (Mouse); 25724 (Rat)
Alternate Names
CD104 antigen; CD104; GP150; Integrin beta 4; integrin beta-4 subunit; integrin beta-4; integrin, beta 4; ITGB4

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Citations for Human Integrin  beta 4/CD104 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Human Basal and Suprabasal Keratinocytes Are Both Able to Generate and Maintain Dermo-Epidermal Skin Substitutes in Long-Term In Vivo Experiments
    Authors: L Pontiggia, AK Ahuja, HK Yosef, D Rütsche, E Reichmann, U Moehrlen, T Biedermann
    Cells, 2022-07-09;11(14):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Plasma membrane proteoglycans syndecan-2 and syndecan-4 engage with EGFR and RON kinase to sustain carcinoma cell cycle progression
    Authors: DM Beauvais, SE Nelson, KM Adams, NA Stueven, O Jung, AC Rapraeger
    The Journal of Biological Chemistry, 2022-05-13;0(0):102029.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  3. Human Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells
    Authors: CR Keller, Y Hu, KF Ruud, AE VanDeen, SR Martinez, BT Kahn, Z Zhang, RK Chen, W Li
    Cancers, 2021-11-22;13(22):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Integrin alpha 5 promotes migration and invasion through the FAK/STAT3/AKT signaling pathway in icotinib‑resistant non‑small cell lung cancer cells
    Authors: Yang Yang, Yizhe Wang, Xiaofang Che, Kezuo Hou, Jie Wu, Chunlei Zheng et al.
    Oncology Letters
  5. Clinical impact of different exosomes' protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapy
    Authors: R Giampieri, F Piva, G Occhipinti, A Bittoni, A Righetti, S Pagliarett, A Murrone, F Bianchi, C Amantini, M Giulietti, G Ricci, G Principato, G Santoni, R Berardi, S Cascinu
    PLoS ONE, 2019-05-02;14(5):e0215990.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Detection
  6. Enteric Species F Human Adenoviruses use Laminin-Binding Integrins as Co-Receptors for Infection of Ht-29 Cells
    Authors: A Rajan, BD Persson, L Frängsmyr, A Olofsson, L Sandblad, J Heino, Y Takada, AP Mould, LM Schnapp, J Gall, N Arnberg
    Sci Rep, 2018-07-03;8(1):10019.
    Applications: Flow Cytometry
  7. Granzyme B is elevated in autoimmune blistering diseases and cleaves key anchoring proteins of the dermal-epidermal junction
    Authors: V Russo, T Klein, DJ Lim, N Solis, Y Machado, S Hiroyasu, L Nabai, Y Shen, MR Zeglinski, H Zhao, CP Oram, PA Lennox, N Van Laeken, NJ Carr, RI Crawford, CW Franzke, CM Overall, DJ Granville
    Sci Rep, 2018-06-26;8(1):9690.
    Applications: Western Blot
  8. Human adenovirus type 37 uses ?V?1 and ?3?1 integrins for infection of human corneal cells
    Authors: Rickard J Storm
    J. Virol, 2017-02-14;0(0):.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-Fr, Neutralization
  9. Integrin alpha6beta4 identifies human distal lung epithelial progenitor cells with potential as a cell-based therapy for cystic fibrosis lung disease.
    Authors: Li X, Rossen N, Sinn P, Hornick A, Steines B, Karp P, Ernst S, Adam R, Moninger T, Levasseur D, Zabner J
    PLoS ONE, 2013-12-12;8(12):e83624.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. Integrin alpha5beta1 facilitates cancer cell invasion through enhanced contractile forces.
    Authors: Mierke CT, Frey B, Fellner M, Herrmann M, Fabry B
    J. Cell. Sci., 2011-01-11;124(0):369-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human Integrin  beta 4/CD104 Antibody

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Human Integrin  beta 4/CD104 Antibody
By Anonymous on 12/13/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma


Human Integrin  beta 4/CD104 Antibody
By Anonymous on 11/10/2018
Application: ELISA Sample Tested: Plasma Species: Human