Human Integrin beta 6 Biotinylated Antibody

Catalog #: BAF4155 Datasheet / COA / SDS

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BAF4155

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Detection of Integrin beta 6 in HT‑29 Human Cell Line by Flow Cytometry.

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Human Integrin beta 6 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin beta 6 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse Integrin beta 6 is observed, approximately 10% cross-reactivity with recombinant human (rh) Integrin beta 3 is observed, and less than 5% cross-reactivity with rhIntegrin beta 5 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Integrin beta 6
Gly22-Asn707
Accession # P18564

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Integrin beta 6

Flow Cytometry

2.5 µg/10⁶ cells

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin beta 6 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Sheep Anti-Human Integrin beta 6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4155, filled histogram) or control antibody (Catalog # BAF020, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

Immunocytochemistry

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Integrin beta 6 in HT‑29 Human Cell Line. Integrin beta 6 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Sheep Anti-Human Integrin beta 6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4155) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 6

Integrin beta 6 (ITG beta 6) is a 95 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha V and appears on epithelia following injury or inflammation. It activates TGF-beta and assists keratinocyte migration. Human Integrin beta 6 is a type I transmembrane glycoprotein that is 767 aa in length. It contains a 688 aa extracellular domain (ECD) (aa 22‑709) that incorporates a 241 aa VWF-A domain. Over aa 22‑707, human integrin beta 6 ECD shares 90% and 93% aa sequence identity with mouse and pig integrin beta 6 ECD, respectively.

Entrez Gene IDs

3694 (Human); 16420 (Mouse); 311061 (Rat); 102122296 (Cynomolgus Monkey)

Alternate Names

Integrin beta 6; integrin beta-6; integrin, beta 6; ITGB6