Human Integrin  beta 6 Antibody

Catalog # Availability Size / Price Qty
AF4155
AF4155-SP
Human Integrin  beta 6 Antibody in Flow Cytometry
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Product Details
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Human Integrin  beta 6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin  beta 6 in the direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant mouse Integrin  beta 6 is observed and less than 5% cross-reactivity with recombinant human (rh) Integrin  beta 3 and rhIntegrin  beta 5 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin beta 6
Gly22-Asn707
Accession # P18564
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Integrin  beta 6
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Integrin  beta 6 in HT‑29 Human Cell Line by Flow Cytometry. View Larger

Detection of Integrin  beta 6 in HT‑29 Human Cell Line by Flow Cytometry. HT‑29 human colon adenocarcinoma cell line was stained with Sheep Anti-Human Integrin  beta 6 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4155, filled histogram) or isotype control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

Immunocytochemistry Integrin  beta 6 in HT‑29 Human Cell Line. View Larger

Integrin  beta 6 in HT‑29 Human Cell Line. Integrin  beta 6 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line using Sheep Anti-Human Integrin  beta 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4155) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 6

Integrin beta 6 (ITG beta 6) is a 95 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha V and appears on epithelia following injury or inflammation. It activates TGF-beta and assists keratinocyte migration. Human Integrin beta 6 is a type I transmembrane glycoprotein that is 767 aa in length. It contains a 688 aa extracellular domain (ECD) (aa 22‑709) that incorporates a 241 aa VWF-A domain. Over aa 22‑707, human integrin beta 6 ECD shares 90% and 93% aa sequence identity with mouse and pig integrin beta 6 ECD, respectively.

Entrez Gene IDs
3694 (Human); 16420 (Mouse); 311061 (Rat); 102122296 (Cynomolgus Monkey)
Alternate Names
Integrin beta 6; integrin beta-6; integrin, beta 6; ITGB6

Product Datasheets

FAQs

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Human Integrin  beta 6 Antibody
By Anonymous on 03/12/2018
Application: ICC/IF Sample Tested: Adult lung Species: Human