Detection of ISG15/UCRP in Jurkat Human Cell Line by Flow Cytometry.
Jurkat human acute T cell leukemia cell line untreated (light orange filled histogram) or treated with 500 U/mL Recombinant Human IFN‑ beta 1a (Mammalian) (Catalog # 11415-1) for 16 hours (dark orange filled histogram) was stained with Rat Anti-Human ISG15/UCRP Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC8044G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
ISG15 (interferon-induced 15 kDa protein; also known as IP17) is a 15 kDa ubiquitin family protein that is expressed by a wide variety of cell types. ISG15 is induced by type-I IFN, and like ubiquitin, becomes conjugated to intracellular proteins. Unlike ubiquitin, it does not target proteins for degradation. Instead, it is part of the antivirus response. Human proISG15 is 165 amino acids (aa) in length. There are two ubiquitin-like domains (aa 2-78 and 79-157) and a propeptide (aa 158-165) that is cleaved intracellularly. Select blood mononuclear cells secrete mature ISG15, which is chemotactic for neutrophils. Mature human ISG15 is 66% aa identical to mouse ISG15.
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