Human ISG15/UCRP APC-conjugated Antibody

Catalog # Availability Size / Price Qty
IC8044A
Detection of ISG15/UCRP in Jurkat Human Cell Line by Flow Cytometry.
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Citations (1)
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Human ISG15/UCRP APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ISG15/UCRP in Elisa and Western Blot.
Source
Monoclonal Rat IgG2A Clone # 851701
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Full-length recombinant human ISG15/UCRP

Accession # P05161
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of ISG15/UCRP antibody in Jurkat Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ISG15/UCRP in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line untreated (light orange filled histogram) or treated with 500 U/mL Recombinant Human IFN-beta 1a (Mammalian) (Catalog # 11415-1) for 16 hours (dark orange filled histogram) was stained with Rat Anti-Human ISG15/UCRP APC-conjugated Monoclonal Antibody (Catalog # IC8044A) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ISG15/UCRP

ISG15 (interferon-induced 15 kDa protein; also IP17) is a 15 kDa ubiquitin family protein that is expressed by a wide variety of cell types. ISG15 is induced by type-I IFN, and like ubiquitin, becomes conjugated to intracellular proteins. Unlike ubiquitin, it does not target proteins for degradation. Instead, it is part of the antivirus response. Human proISG15 is 165 amino acids (aa) in length. There are two ubiquitin-like domains (aa 2-78 and 79-157) and a propeptide (aa 158-165) that is cleaved intracellularly. Select blood mononuclear cells secrete mature ISG15, which is chemotactic for neutrophils. Mature human ISG15 is 66% aa identical to mouse ISG15.

Long Name
ISG15 Ubiquitin-like Modifier
Entrez Gene IDs
9636 (Human); 100038882 (Mouse); 298693 (Rat)
Alternate Names
G1P2; G1P2interferon-induced 17-kDa/15-kDa protein; IFI15; IFI15hUCRP; interferon, alpha-inducible protein (clone IFI-15K); Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; interferon-stimulated protein, 15 kDa; IP17; ISG15 ubiquitin-like modifier; ISG15; Ubiquitin cross-reactive protein; ubiquitin-like protein ISG15; UCRP; UCRPIP17

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Citation for Human ISG15/UCRP APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Human-Specific Adaptations in Vpu Conferring Anti-tetherin Activity Are Critical for Efficient Early HIV-1 Replication In�Vivo
    Authors: E Yamada, S Nakaoka, L Klein, E Reith, S Langer, K Hopfensper, S Iwami, G Schreiber, F Kirchhoff, Y Koyanagi, D Sauter, K Sato
    Cell Host Microbe, 2018;23(1):110-120.e7.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot

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