Human KIR/CD158 Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1848F
Detection of KIR/CD158 in HumanPBMCs by Flow Cytometry.
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Citations (2)
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Human KIR/CD158 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human KIR/CD158. Stains cells transfected with KIR2DL2, 2DL3, 2DS2, or 2DS4. It does not stain cells transfected with KIR2DL1, 2DL4, 3DL1, or 3DL2.
Source
Monoclonal Mouse IgG2B Clone # 180704
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human KIR2DL3
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of KIR/CD158 in HumanPBMCs by Flow Cytometry. View Larger

Detection of KIR/CD158 in HumanPBMCs by Flow Cytometry. Human peripheral blood mononuclearcells (PBMCs) were stained with Mouse Anti-Human KIR/CD158Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1848F) andMouse Anti-Human NCAM‑1/CD56 PE‑conjugated MonoclonalAntibody (Catalog # FAB2408P).View our protocol for StainingMembrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KIR/CD158

KIR is a family of immunoglobulin-like receptors expressed on NK cells and some T cells. Family members with long (L) cytoplasmic tails deliver inhibitory signals and those with short (S) cytoplasmic tails deliver activating signals. Several KIR proteins are known to recognize particular alleles of MHC class I proteins.

Alternate Names
CD158 antigen-like family member A; CD158; CD158a antigen; CD158Ankat1; cl-42; killer cell immunoglobulin-like receptor 2DL1; killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 1; killer Ig receptor; killer inhibitory receptor 2-2-1; KIR; KIR221; MHC class I NK cell receptor; Natural killer-associated transcript 1; NKAT; NKAT-1; NKAT1KIR-K64; p58 killer cell inhibitory receptor KIR-K64; p58 natural killer cell receptor clones CL-42/47.11; p58 NK cell inhibitory receptor NKR-K6,47.11; p58 NK receptor CL-42/47.11; p58.1 MHC class-I-specific NK receptor; p58.1

Product Datasheets

Citations for Human KIR/CD158 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Eomesodermin and T-bet mark developmentally distinct human natural killer cells
    Authors: A Collins, N Rothman, K Liu, SL Reiner
    JCI Insight, 2017;2(5):e90063.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. IL-12 directs further maturation of ex vivo differentiated NK cells with improved therapeutic potential.
    Authors: Lehmann, Dorit, Spanholtz, Jan, Sturtzel, Caterina, Tordoir, Marleen, Schlechta, Bernhard, Groenewegen, Dirk, Hofer, Erhard
    PLoS ONE, 2014;9(1):e87131.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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