Human KIR/CD158 Antibody

Catalog # Availability Size / Price Qty
MAB1848
MAB1848-SP
Detection of KIR/CD158 in PBMC lymphocytes by Flow Cytometry
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Product Details
Citations (2)
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Human KIR/CD158 Antibody Summary

Species Reactivity
Human
Specificity
Detects human KIR/CD158. Stains cells transfected with KIR2DL2, 2DL3, 2DS2, or 2DS4. It does not stain cells transfected with KIR2DL1, 2DL4, 3DL1, or 3DL2.
Source
Monoclonal Mouse IgG2B Clone # 180704
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human KIR2DL3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.1 µg/106 cells
Human peripheral blood CD56+ natural killer cells
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of KIR/CD158 in PBMC lymphocytes by Flow Cytometry PBMC lymphocytes were stained with Rabbit Anti-Human NCAM‑1/CD56 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB24086N) and either (A) Mouse Anti-Human KIR/CD158 Monoclonal Antibody (Catalog # MAB1848) or (B) isotype control antibody (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KIR/CD158

KIR is a family of immunoglobulin-like receptors expressed on NK cells and some T cells. Family members with long (L) cytoplasmic tails deliver inhibitory signals and those with short (S) cytoplasmic tails deliver activating signals. Several KIR proteins are known to recognize particular alleles of MHC class I proteins.

Alternate Names
CD158 antigen-like family member A; CD158; CD158a antigen; CD158Ankat1; cl-42; killer cell immunoglobulin-like receptor 2DL1; killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 1; killer Ig receptor; killer inhibitory receptor 2-2-1; KIR; KIR221; MHC class I NK cell receptor; Natural killer-associated transcript 1; NKAT; NKAT-1; NKAT1KIR-K64; p58 killer cell inhibitory receptor KIR-K64; p58 natural killer cell receptor clones CL-42/47.11; p58 NK cell inhibitory receptor NKR-K6,47.11; p58 NK receptor CL-42/47.11; p58.1 MHC class-I-specific NK receptor; p58.1

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Citations for Human KIR/CD158 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. HLA-C and KIR permutations influence chronic obstructive pulmonary disease risk
    Authors: T Mkorombind, TK Tran-Nguye, K Yuan, Y Zhang, J Xue, GJ Criner, YI Kim, JM Pilewski, A Gaggar, MH Cho, FC Sciurba, SR Duncan
    JCI Insight, 2021-10-08;6(19):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  2. A one year follow-up study of natural killer and dendritic cells activities in multiple sclerosis patients receiving glatiramer acetate (GA).
    Authors: Hoglund R, Holmoy T, Harbo H, Maghazachi A
    PLoS ONE, 2013-04-22;8(4):e62237.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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