Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry, CyTof, Functional Assay, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 180704
View all KIR/CD158 Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human KIR2DL3

Specificity

Detects human KIR/CD158. Stains cells transfected with KIR2DL2, 2DL3, 2DS2, or 2DS4. It does not stain cells transfected with KIR2DL1, 2DL4, 3DL1, or 3DL2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human KIR/CD158 Antibody

Detection of KIR/CD158 in PBMC lymphocytes by Flow Cytometry

PBMC lymphocytes were stained with Rabbit Anti-Human NCAM‑1/CD56 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB24086N) and either (A) Mouse Anti-Human KIR/CD158 Monoclonal Antibody (Catalog # MAB1848) or (B) isotype control antibody (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human KIR/CD158 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.1 µg/106 cells
Sample: Human peripheral blood CD56+ natural killer cells

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KIR/CD158

KIR is a family of immunoglobulin-like receptors expressed on NK cells and some T cells. Family members with long (L) cytoplasmic tails deliver inhibitory signals and those with short (S) cytoplasmic tails deliver activating signals. Several KIR proteins are known to recognize particular alleles of MHC class I proteins.

Alternate Names

CD158

Additional KIR/CD158 Products

Product Documents for Human KIR/CD158 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human KIR/CD158 Antibody

For research use only

Citations for Human KIR/CD158 Antibody

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FAQs

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