hh Human KIR2DL3/CD158b2 Fluorescein-conjugated Antibody FAB2014F-025: R&D Systems

Human KIR2DL3/CD158b2 Fluorescein-conjugated Antibody

(7 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human KIR2DL3/CD158b2. Stains BaF3 cells transfected with human KIR2DL3 in flow cytometry. Does not stain BaF3 cells transfected with KIR2DL1, 2DL2, 2DL4, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1.
  • Source
    Monoclonal Mouse IgG2A Clone # 180701
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human KIR2DL3/CD158b2
    Accession # P43628
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of KIR2DL3/CD158b2 inHuman PBMCs by Flow Cytometry. Human peripheral blood mononuclearcells (PBMCs) were stained with Mouse Anti-Human KIR2DL3/CD158b2Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB2014F) andMouse Anti-Human NCAM‑1/CD56 PE‑conjugated MonoclonalAntibody (Catalog # FAB2408P).View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: KIR2DL3/CD158b2

KIR2DL3 is one of several immunoglobulin-like receptors expressed on NK and T cells that bind MHC class I molecules and regulate killer cell activity. KIR2DL3 interacts specifically with HLA-Cw3.

  • Long Name:
    Killer Cell Immunoglobulin-like Receptor, Two Domain Long Cytoplasmic Tail, 3
  • Entrez Gene IDs:
    3804 (Human)
  • Alternate Names:
    CD158 antigen-like family member B2; CD158b2 antigen; CD158b2; CD158B2CD158b; cl-6; killer cell immunoglobulin-like receptor 2DL3; killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 3; Killer inhibitory receptor cl 2-3; KIR-023GB; KIR2DL3; KIRCL23; KIR-K7b; KIR-K7c; MGC129943; MHC class I NK cell receptor; natural killer associated transcript 2; natural killer cell inhibitory receptor KIR2DL3; Natural killer-associated transcript 2; NKAT; NKAT2; NKAT-2; NKAT2A; nkat2b; NKAT2GL183; NK-receptor; p58 natural killer cell receptor clone CL-6; p58 NK receptor CL-6; p58; p58.2 MHC class-I specific NK receptor; p58.2 MHC class-I-specific NK receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. 2DL1, 2DL2 and 2DL3 all contribute to KIR phenotype variability on human NK cells.
    Authors: Dunphy S, Guinan K, Chorcora C, Jayaraman J, Traherne J, Trowsdale J, Pende D, Middleton D, Gardiner C
    Genes Immun, 2015;16(5):301-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells.
    Authors: Marquardt N, Beziat V, Nystrom S, Hengst J, Ivarsson M, Kekalainen E, Johansson H, Mjosberg J, Westgren M, Lankisch T, Wedemeyer H, Ellis E, Ljunggren H, Michaelsson J, Bjorkstrom N
    J Immunol, 2015;194(6):2467-71.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT.
    Authors: Holmes T, Wilson E, Black E, Benest A, Vaz C, Tan B, Tanavde V, Cook G
    Proc Natl Acad Sci U S A, 2014;111(52):E5688-96.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Modulation of the natural killer cell KIR repertoire by cytomegalovirus infection.
    Authors: Charoudeh H, Terszowski G, Czaja K, Gonzalez A, Schmitter K, Stern M
    Eur J Immunol, 2013;43(2):480-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Molecular characterisation of KIR2DS2*005, a fusion gene associated with a shortened KIR haplotype.
    Authors: Ordonez D, Gomez-Lozano N, Rosales L, Vilches C
    Genes Immun., 2011;12(7):544-51.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus.
    Authors: Bjorkstrom NK, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, Michaelsson J, Malmberg KJ, Klingstrom J, Ahlm C, Ljunggren HG
    J. Exp. Med., 2011;208(1):13-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. NK cell terminal differentiation: correlated stepwise decrease of NKG2A and acquisition of KIRs.
    Authors: Beziat V, Descours B, Parizot C, Debre P, Vieillard V
    PLoS ONE, 2010;5(8):e11966.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Fluorescein-conjugated Antibody

Ctrl IC003F 6  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Flow Cytometry Human KIR2DL3/CD158b2 Fluorescein-conjugated Antibody FAB2014F
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 Flow Human Anonymous 07/17/2017
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Flow Cytometry Human KIR2DL3/CD158b2 Fluorescein-conjugated Antibody FAB2014F
Flow Cytometry: Human KIR2DL3/CD158b2 Fluorescein-conjugated Antibody [FAB2014F]

Summary

ApplicationFlow Cytometry
Sample TestedPeripheral blood mononuclear cells (PBMCs)
SpeciesHuman

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