Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-reported

Cited:

Flow Cytometry, CyTof, Flow Cytometry Control, CyTOF-reported

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 180701
View all KIR2DL3/CD158b2 Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human KIR2DL3/CD158b2
Accession # P43628

Specificity

Detects human KIR2DL3/CD158b2. Stains BaF3 cells transfected with human KIR2DL3 in flow cytometry. Does not stain BaF3 cells transfected with KIR2DL1, 2DL2, 2DL4, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human KIR2DL3/CD158b2 Antibody

Detection of KIR2DL3/CD158b2 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of KIR2DL3/CD158b2 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human KIR2DL3/CD158b2 Monoclonal Antibody (Catalog # MAB2014) or (B) Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P).View our protocol for Staining Membrane-associated Proteins.

Applications for Human KIR2DL3/CD158b2 Antibody

Application
Recommended Usage

CyTOF-reported

Horowitz, A. et al. (2015) J. Immunol. 195: 4524. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human whole blood CD56+ natural killer cells

Reviewed Applications

Read 1 review rated 4 using MAB2014 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KIR2DL3/CD158b2

KIR2DL3 is one of several immunoglobulin-like receptors expressed on NK and T cells that bind MHC class I molecules and regulate killer cell activity. KIR2DL3 interacts specifically with HLA-Cw3.

Long Name

Killer Cell Immunoglobulin-like Receptor, Two Domain Long Cytoplasmic Tail, 3

Alternate Names

CD158b2, NKAT2

Entrez Gene IDs

3804 (Human)

Gene Symbol

KIR2DL3

UniProt

P43628

Additional KIR2DL3/CD158b2 Products

Product Documents for Human KIR2DL3/CD158b2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human KIR2DL3/CD158b2 Antibody

For research use only

Citations for Human KIR2DL3/CD158b2 Antibody

Customer Reviews for Human KIR2DL3/CD158b2 Antibody (1)

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  • Human KIR2DL3/CD158b2 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: HT1080 human fibrosarcoma cell line
    Species: Human
    Verified Customer | Posted 06/15/2018

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