Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 179315
View all KIR2DS4/CD158i Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human KIR2DS4
Accession # P43632

Specificity

Detects KIR2DS4/CD158i in Western blots. In flow cytometry, this antibody stains cells transfected with KIR2DS4/CD158i. Does not stain cells transfected with KIR2DL1, 2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 3DL1, 3DL2, or 3DS1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human KIR2DS4/CD158i APC‑conjugated Antibody

Detection of KIR2DS4/CD158i antibody in Human PBMCs antibody by Flow Cytometry.

Detection of KIR2DS4/CD158i in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human KIR2DS4/CD158i APC‑conjugated Monoclonal Antibody (Catalog # FAB1847A) and Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human KIR2DS4/CD158i APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KIR2DS4/CD158i

KIR2DS4 is an activating immunoglobulin-like receptor expressed on NK cells and some T cells. It has been implicated in the recognition of some HLA-C alleles (1). A deletion variant encoding a single domain secreted isoform of KIR2DS4 has also been described (2).

References

  1. Katz, G. et al. (2001) J. Immunol. 166:7260.
  2. Maxwell, L. et al. (2002) Tissue Antigens 60:254.

Long Name

Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 4

Alternate Names

CD158i

Entrez Gene IDs

3809 (Human)

Gene Symbol

KIR2DS4

UniProt

P43632

Additional KIR2DS4/CD158i Products

Product Documents for Human KIR2DS4/CD158i APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human KIR2DS4/CD158i APC‑conjugated Antibody

For research use only

Citations for Human KIR2DS4/CD158i APC‑conjugated Antibody

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