Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry, Functional Assay, CyTOF-reported

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 179315
View all KIR2DS4/CD158i Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human KIR2DS4
Accession # P43632

Specificity

Detects KIR2DS4/CD158i in Western blots. In flow cytometry, this antibody stains cells transfected with KIR2DS4/CD158i. Does not stain cells transfected with KIR2DL1, 2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 3DL1, 3DL2, or 3DS1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human KIR2DS4/CD158i Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human whole blood CD56+ natural killer cells

Western Blot

1 µg/mL
Sample: Recombinant Human KIR2DS4 under non-reducing conditions only

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KIR2DS4/CD158i

KIR2DS4 is an activating immunoglobulin-like receptor expressed on NK cells and some T cells. It has been implicated in the recognition of some HLA-C alleles (1). A deletion variant encoding a single domain secreted isoform of KIR2DS4 has also been described (2).

References

  1. Katz, G. et al. (2001) J. Immunol. 166:7260.
  2. Maxwell, L. et al. (2002) Tissue Antigens 60:254.

Long Name

Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 4

Alternate Names

CD158i

Entrez Gene IDs

3809 (Human)

Gene Symbol

KIR2DS4

UniProt

P43632

Additional KIR2DS4/CD158i Products

Product Documents for Human KIR2DS4/CD158i Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human KIR2DS4/CD158i Antibody

For research use only

Citations for Human KIR2DS4/CD158i Antibody

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