Human KIR2DS4/CD158i Antibody

Catalog # Availability Size / Price Qty
MAB1847
MAB1847-SP
Product Details
Citations (3)
FAQs
Supplemental Products
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Human KIR2DS4/CD158i Antibody Summary

Species Reactivity
Human
Specificity
Detects KIR2DS4/CD158i in Western blots. In flow cytometry, this antibody stains cells transfected with KIR2DS4/CD158i. Does not stain cells transfected with KIR2DL1, 2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 3DL1, 3DL2, or 3DS1.
Source
Monoclonal Mouse IgG2A Clone # 179315
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human KIR2DS4
Accession # P43632
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Complete Your Experiment

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human KIR2DS4 under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
Human whole blood CD56+ natural killer cells
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KIR2DS4/CD158i

KIR2DS4 is an activating immunoglobulin-like receptor expressed on NK cells and some T cells. It has been implicated in the recognition of some HLA-C alleles (1). A deletion variant encoding a single domain secreted isoform of KIR2DS4 has also been described (2).

References
  1. Katz, G. et al. (2001) J. Immunol. 166:7260.
  2. Maxwell, L. et al. (2002) Tissue Antigens 60:254.
Long Name
Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 4
Entrez Gene IDs
3809 (Human)
Alternate Names
CD158 antigen-like family member I; CD158i antigen; CD158i; cl-39; killer cell immunoglobulin-like receptor 2DS4; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail4,nkat8; killer inhibitory receptor 4-1-2; KIR antigen 2DS4; KIR2DS4; KKA3KIR1D; MGC120019; MGC125315; MGC125317; MHC class I NK cell receptor; natural killer cell inhibitory receptor; Natural killer-associated transcript 8; NKAT-8; NKAT8KIR412; P58 natural killer cell receptor clones CL-39/CL-17; p58 NK receptor CL-39/CL-17

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Citations for Human KIR2DS4/CD158i Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Pro- and Anti-Inflammatory Cytokines in the Context of NK Cell-Trophoblast Interactions
    Authors: V Mikhailova, P Grebenkina, E Khokhlova, A Davydova, Z Salloum, E Tyshchuk, V Zagainova, K Markova, I Kogan, S Selkov, D Sokolov
    International Journal of Molecular Sciences, 2022;23(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Human NK cell receptor KIR2DS4 detects a conserved bacterial epitope presented by HLA-C
    Authors: MJW Sim, S Rajagopala, DM Altmann, RJ Boyton, PD Sun, EO Long
    Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  3. Activating KIR2DS4 Is Expressed by Uterine NK Cells and Contributes to Successful Pregnancy
    Authors: Ashley Moffett
    J. Immunol., 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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