Human KLF12 Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
Detection of KLF12 in MG‑63 Human Cell Line by Flow Cytometry.
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Product Details
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Human KLF12 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Detects human KLF12 in direct ELISAs.
Recombinant Monoclonal Rabbit IgG Clone # 1230C
Protein A or G purified from cell culture supernatant
Mouse myeloma cell line NS0-derived human KLF12
Accession # Q9Y4X4
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)


Recommended Concentration
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of KLF12 antibody in MG-63 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of KLF12 in MG‑63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Rabbit Anti-Human KLF12 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC9097R, filled histogram) or isotype control antibody (Catalog # IC1051R, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KLF12

KLF12 (Kruppel-like factor 12), also known as AP-2rep and AP-2 alpha, is a 50-55 kDa member of the Sp1 C2H2-type zinc-finger protein family of transcription factors. Its alternate name, AP-2 alpha, should not be confused with the AP-2 alpha factor described by SwissProt # P05549. The molecule's name derivers from it structural similarity to fruitfly Kruppel. "Kruppel" being a German word meaning "cripple", a phenotype observed in larva derived from Kruppel knock-out flies. KLF12 is 402 amino acids (aa) in length and characterized by the presence of an N-terminal PVDLS (ProValAspLeuSer) motif coupled to three C2H2-type zinc-finger domains. This classifies it as a KLF3/8/12 subfamily member. KLF is described as being a transcriptional repressor that binds to G-C rich areas of DNA. Cells known to express KLF12 include corneal epithelium, vascular endothelium and renal collecting duct epithelium. Full-length human KLF12 shares 97% aa sequence identity with mouse KLF12.

Long Name
Kruppel-Like Factor 12
Entrez Gene IDs
11278 (Human); 16597 (Mouse); 306110 (Rat)
Alternate Names
AP-2rep transcription factor; AP2REP; AP-2rep; AP2REPAP-2 repressor; HSPC122; KLF12 zinc finger transcriptional repressor; KLF12; Krueppel-like factor 12; Kruppel-like factor 12; Transcriptional repressor AP-2rep

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.


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