Human KLF12 Alexa Fluor® 647-conjugated Antibody Summary
Accession # Q9Y4X4
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of KLF12 in MG‑63 Human Cell Line by Flow Cytometry. MG‑63 human osteosarcoma cell line was stained with Rabbit Anti-Human KLF12 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC9097R, filled histogram) or isotype control antibody (Catalog # IC1051R, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
KLF12 (Kruppel-like factor 12), also known as AP-2rep and AP-2 alpha, is a 50-55 kDa member of the Sp1 C2H2-type zinc-finger protein family of transcription factors. Its alternate name, AP-2 alpha, should not be confused with the AP-2 alpha factor described by SwissProt # P05549. The molecule's name derivers from it structural similarity to fruitfly Kruppel. "Kruppel" being a German word meaning "cripple", a phenotype observed in larva derived from Kruppel knock-out flies. KLF12 is 402 amino acids (aa) in length and characterized by the presence of an N-terminal PVDLS (ProValAspLeuSer) motif coupled to three C2H2-type zinc-finger domains. This classifies it as a KLF3/8/12 subfamily member. KLF is described as being a transcriptional repressor that binds to G-C rich areas of DNA. Cells known to express KLF12 include corneal epithelium, vascular endothelium and renal collecting duct epithelium. Full-length human KLF12 shares 97% aa sequence identity with mouse KLF12.
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