Human KLF12 Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody..
  • Species Reactivity
  • Specificity
    Detects human KLF12 in direct ELISAs.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1230C
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived human KLF12
    Accession # Q9Y4X4
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of KLF12 in MG‑63 Human Cell Line by Flow Cytometry. MG‑63 human osteosarcoma cell line was stained with Rabbit Anti-Human KLF12 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC9097R, filled histogram) or isotype control antibody (Catalog # IC1051R, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: KLF12
KLF12 (Kruppel-like factor 12), also known as AP-2rep and AP-2 alpha, is a 50-55 kDa member of the Sp1 C2H2-type zinc-finger protein family of transcription factors. Its alternate name, AP-2 alpha, should not be confused with the AP-2 alpha factor described by SwissProt # P05549. The molecule's name derivers from it structural similarity to fruitfly Kruppel. "Kruppel" being a German word meaning "cripple", a phenotype observed in larva derived from Kruppel knock-out flies. KLF12 is 402 amino acids (aa) in length and characterized by the presence of an N-terminal PVDLS (ProValAspLeuSer) motif coupled to three C2H2-type zinc-finger domains. This classifies it as a KLF3/8/12 subfamily member. KLF is described as being a transcriptional repressor that binds to G-C rich areas of DNA. Cells known to express KLF12 include corneal epithelium, vascular endothelium and renal collecting duct epithelium. Full-length human KLF12 shares 97% aa sequence identity with mouse KLF12.
  • Long Name:
    Kruppel-Like Factor 12
  • Entrez Gene IDs:
    11278 (Human); 16597 (Mouse); 306110 (Rat)
  • Alternate Names:
    AP-2rep transcription factor; AP2REP; AP-2rep; AP2REPAP-2 repressor; HSPC122; KLF12 zinc finger transcriptional repressor; Krueppel-like factor 12; Kruppel-like factor 12; Transcriptional repressor AP-2rep
Related Research Areas
Isotype Controls
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Rabbit IgG Alexa Fluor® 647-conjugated Antibody

Ctrl IC1051R
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