Human KLF12 Alexa Fluor® 594-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC9097T-025
Detection  of KLF12 in MG‑63 Human Cell Line by Flow Cytometry.
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Human KLF12 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human KLF12 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1230C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived human KLF12
Asn2-Val402
Accession # Q9Y4X4
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection  of KLF12 in MG‑63 Human Cell Line by Flow Cytometry. View Larger

Detection of KLF12 in MG‑63 Human Cell Line by Flow Cytometry. MG‑63 human osteosarcoma cell line was stained with Rabbit Anti-Human KLF12 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # IC9097T, filled histogram) or isotype control antibody (Catalog # IC1051T, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KLF12

KLF12 (Kruppel-like factor 12), also known as AP-2rep and AP-2 alpha, is a 50-55 kDa member of the Sp1 C2H2-type zinc-finger protein family of transcription factors. Its alternate name, AP-2 alpha, should not be confused with the AP-2 alpha factor described by SwissProt # P05549. The molecule's name derivers from it structural similarity to fruitfly Kruppel. "Kruppel" being a German word meaning "cripple", a phenotype observed in larva derived from Kruppel knock-out flies. KLF12 is 402 amino acids (aa) in length and characterized by the presence of an N-terminal PVDLS (ProValAspLeuSer) motif coupled to three C2H2-type zinc-finger domains. This classifies it as a KLF3/8/12 subfamily member. KLF is described as being a transcriptional repressor that binds to G-C rich areas of DNA. Cells known to express KLF12 include corneal epithelium, vascular endothelium and renal collecting duct epithelium. Full-length human KLF12 shares 97% aa sequence identity with mouse KLF12.

Long Name
Kruppel-Like Factor 12
Entrez Gene IDs
11278 (Human); 16597 (Mouse); 306110 (Rat)
Alternate Names
AP-2rep transcription factor; AP2REP; AP-2rep; AP2REPAP-2 repressor; HSPC122; KLF12 zinc finger transcriptional repressor; KLF12; Krueppel-like factor 12; Kruppel-like factor 12; Transcriptional repressor AP-2rep

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