Human KLF12 Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human KLF12 in direct ELISAs.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1230C
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived human KLF12
    Asn2-Val402
    Accession # Q9Y4X4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    1-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
KLF12 in HEK293 Human Cell Line. KLF12 was detected in immersion fixed HEK293 human embryonic kidney cell line using Rabbit Anti-Human KLF12 Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB9097) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Intracellular Staining by Flow Cytometry
Detection of KLF12 in MG‑63 Human Cell Line by Flow Cytometry. MG‑63 human osteosarcoma cell line was stained with Rabbit Anti-Human KLF12 Monoclonal Antibody (Catalog # MAB9097, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody
(Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: KLF12
Krueppel-like factor 12 is a protein that in humans is encoded by the KLF12 gene. The protein encoded by this gene confers strong transcriptional repression to the AP-2-alpha gene, a developmentally-regulated transcription factor active during vertebrate development and carcinogenesis. KLF12 has been reported as a novel tumor-suppressor gene whose loss of function is associated with anoikis resistance. Three isoforms have been found for this gene.
  • Long Name:
    Kruppel-Like Factor 12
  • Entrez Gene IDs:
    11278 (Human); 16597 (Mouse); 306110 (Rat)
  • Alternate Names:
    AP-2rep transcription factor; AP2REP; AP-2rep; AP2REPAP-2 repressor; HSPC122; KLF12 zinc finger transcriptional repressor; KLF12; Krueppel-like factor 12; Kruppel-like factor 12; Transcriptional repressor AP-2rep
Related Research Areas
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Isotype Controls
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Normal Rabbit IgG Control

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Normal Rabbit IgG Control

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Secondary Antibodies
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

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Rabbit IgG APC-conjugated Antibody

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Rabbit IgG PE-conjugated Antibody

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Rabbit IgG Fluorescein-conjugated Antibody

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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Rabbit IgG Antibody

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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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