Human LAG‑3 PerCP‑conjugated Antibody

  • Specificity
    Detects human LAG-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse LAG-3 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human LAG-3
    Accession # P18627
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of LAG‑3 in Human CD3+ PBMCs by Flow Cytometry. CD3+ human peripheral blood mononuclear cells (PBMCs) were either (A) untreated or (B) treated with 1 μg/mL PHA for 5 days were stained with Goat Anti-Human LAG‑3 PerCP‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2319C) and Mouse Anti-Human CD3 epsilon Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB100F). Quadrant markers were set based on control antibody staining (Catalog # IC108C). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LAG-3

LAG-3 (Lymphocyte Activation Gene-3), also known as CD223, is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. LAG-3 and CD4 molecules share < 20% aa sequence homology but have a similar structure (1, 2). Both molecules bind to MHC class II. LAG-3 binds to MHC class II with higher affinity compared to CD4. Both LAG-3 and CD4 genes are located on the distal part of the short arm of chromosome 12.

LAG-3 is an activation-induced molecule, expressed on activated T cells and NK cells, but not on resting T cells. Studies using LAG-3 -/- mice have shown significant delay of T cell apoptosis following antigen stimulation and increased size of memory T cells pool following infection (3, 4). It also has been reported that anti-LAG-3 antibodies up-regulate T cell activation by blocking interaction of LAG-3 and MHC class II. The study has demonstrated that LAG-3 is selectively expressed on activated CD4+CD25+ TReg cells and plays a role in their suppressive activity (5). This evidence indicated, unlike the interaction of CD4 with MHC class II that plays a positive role in T cell activation, LAG-3 binds to MHC class II and negatively regulates T cell activation through LAG-3 signaling. On the other hand, studies have shown that binding of LAG-3 to MHC class II molecules on antigen presenting cells induce maturation of dendritic cells and cytokine secretion by monocytes through MHC class II signal transduction (6). Taken together, LAG-3 may have two major functions, it negatively regulates T cells activation through LAG-3 signaling and stimulates antigen presenting cells which express MHC class II.

  • References:
    1. Triebel, F. et al. (1990) J. Exp. Med. 171:1393.
    2. Baixeras, E. et al. (1992) J. Exp. Med 176:327.
    3. Workman, C.J. and D.A. Vignali (2003) Eur. J. Immunol. 33:970.
    4. Workman, C.J. et al. (2004) J. Immunol. 172:5450.
    5. Huang, C.T. et al. (2004) Immunity 21:503.
    6. Andreae, S. et al. (2003) Blood 102:2130.
  • Long Name:
    Lymphocyte-activation Gene 3
  • Alternate Names:
    CD223; CD223; CD223 antigen; FDC; LAG-3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Protein FDC
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