Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry

Cited:

Neutralization

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all LAIR1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LAIR1 isoform 1
Gln22-His163 (predicited)
Accession # Q6GTX8

Specificity

Detects human LAIR1 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human LAIR2 and recombinant mouse LAIR1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human LAIR1 Biotinylated Antibody

Detection of LAIR1 in PBMC lymphocytes by Flow Cytometry

PBMC lymphocytes were stained with Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Goat Anti-Human LAIR1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2664) or (B) isotype control antibody (Catalog # BAF108) followed by Streptavidin-Allophycocyanin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Applications for Human LAIR1 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMC lymphocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Human LAIR1

Reviewed Applications

Read 1 review rated 5 using BAF2664 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LAIR1

Leukocyte-associated Ig-like receptor-1 (LAIR1; also CD305) is a 46 kDa inhibitory receptor belonging to the Ig superfamily. It is a type I transmembrane protein with one extracellular Ig-like domain and two cytoplasmic ITIMs. Four LAIR-1 splice variants exist. LAIR1b has a 17 aa deletion outside the Ig loop in the extracellular domain. It differs from LAIR1c by one aa residue. LAIR1d has a 77 aa truncation in the cytoplasmic domain. LAIR1 is expressed on NK cells, T cells, B cells, monocytes, dendritic cells and most thymocytes. The extracellular domain of human LAIR1 shares 40% aa identity with that of the mouse protein.

Long Name

Leukocyte-associated Ig-like Receptor 1

Alternate Names

CD305, HLAIR1, LAIR-1

Entrez Gene IDs

3903 (Human); 52855 (Mouse); 574531 (Rat); 102136964 (Cynomolgus Monkey)

Gene Symbol

LAIR1

UniProt

Q6GTX8

Additional LAIR1 Products

Product Documents for Human LAIR1 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LAIR1 Biotinylated Antibody

For research use only

Customer Reviews for Human LAIR1 Biotinylated Antibody (1)

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  • Human LAIR1 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 11/29/2022
    Human LAIR1 Biotinylated Antibody BAF2664

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