Human Laminin  alpha 3/Laminin-5 Antibody

Catalog # Availability Size / Price Qty
MAB2144
MAB2144-SP
Laminin  alpha 3/Laminin-5 in MCF 10A Human Cell Line.
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (2)

Human Laminin  alpha 3/Laminin-5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Laminin alpha 3/Laminin‑5 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # P3H9-2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human keratinocytes
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Wayner, E.A. (1993) J. Cell Biol. 121(5):1141; Gallatin, W.M. et al. (1989) Proc. Natl. Acad. Sci. USA 86(12):4654. This application was not tested by R&D Systems.
 
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
Gallatin, W.M. et al. (1989) Proc. Natl. Acad. Sci. 86:4654.
 
Adhesion Blockade
Gonzales, M. et al. (1999) Mol. Biol. Cell 10:259.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry Laminin  alpha 3/Laminin-5 in MCF 10A Human Cell Line. View Larger

Laminin  alpha 3/Laminin-5 in MCF 10A Human Cell Line. Laminin  alpha 3/Laminin-5 was detected in immersion fixed MCF 10A human breast epithelial cell line using 10 µg/mL Mouse Anti-Human Laminin alpha 3/Laminin-5 Monoclonal Antibody (Catalog # MAB2144) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Laminin  alpha 3/Laminin‑5 in Human Skin. View Larger

Laminin alpha 3/Laminin‑5 in Human Skin. Laminin alpha 3/Laminin‑5 was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human Laminin alpha 3/Laminin‑5 Monoclonal Antibody (Catalog # MAB2144) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to cell in the epiderimis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laminin alpha 3/Laminin-5

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with Integrins, Dystroglycan and other receptors, Laminins contribute to cell differentiation, cell shape and migration, and maintenance of tissue phenotypes and survival (1). Laminin-5, also known as Epiligrin, consists of an alpha 3, beta 3, and gamma 2 chain. It is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa and other epithelial glands and plays an important role in the initiation and maintenance of epithelial cell anchorage to the underlying connective tissue (2, 3).

References
  1. Colognato, H. et al. (2000) Dev. Dyn. 218:213.
  2. Stahl, S. et al. (1997) J. Cell Sci. 110:55.
  3. Gonzales, M. et al. (1999) Mol. Biol. Cell 10:259.
Entrez Gene IDs
3909 (Human)
Alternate Names
Epiligrin; LAMA3; Laminin alpha 3; Laminin5; Laminin-5

Product Datasheets

Citation for Human Laminin  alpha 3/Laminin-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Elastin fragments drive disease progression in a murine model of emphysema.
    Authors: Houghton AM, Quintero PA, Perkins DL, Kobayashi DK, Kelley DG, Marconcini LA, Mecham RP, Senior RM, Shapiro SD
    J. Clin. Invest., 2006;116(3):753-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Neut

FAQs

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Cell and Tissue Staining Kits

ExactaChIP Chromatin Immunoprecipitation Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG1 Isotype Control

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Reconstitution Buffers

Secondary Antibodies

Reviews for Human Laminin  alpha 3/Laminin-5 Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human Laminin  alpha 3/Laminin-5 Antibody
By Anonymous on 05/08/2018
Application: Works very well! Sample Tested: Eye lens tissue Species: Rabbit

Human Laminin  alpha 3/Laminin-5 Antibody
By Anonymous on 04/26/2017
Application: ICC/IF Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human