Laminin alpha 3/Laminin-5 in MCF 10A Human Cell Line.
Laminin alpha 3/Laminin-5 was detected in immersion fixed MCF 10A human breast epithelial cell line using 10 µg/mL Mouse Anti-Human Laminin alpha 3/Laminin-5 Monoclonal Antibody (Catalog # MAB2144) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Laminin alpha 3/Laminin‑5 in Human Skin.
Laminin alpha 3/Laminin‑5 was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human Laminin alpha 3/Laminin‑5 Monoclonal Antibody (Catalog # MAB2144) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to cell in the epiderimis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Laminin alpha 3/Laminin-5
Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with Integrins, Dystroglycan and other receptors, Laminins contribute to cell differentiation, cell shape and migration, and maintenance of tissue phenotypes and survival (1). Laminin-5, also known as Epiligrin, consists of an alpha 3, beta 3, and gamma 2 chain. It is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa and other epithelial glands and plays an important role in the initiation and maintenance of epithelial cell anchorage to the underlying connective tissue (2, 3).
Colognato, H. et al. (2000) Dev. Dyn. 218:213.
Stahl, S. et al. (1997) J. Cell Sci. 110:55.
Gonzales, M. et al. (1999) Mol. Biol. Cell 10:259.
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We have 2 reviews tested in 2 applications: Works very well!, Immunocytochemistry/Immunofluorescence.