Human Langerin/CD207 Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Langerin/CD207 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 343828
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Langerin/CD207
    Tyr64-Pro328
    Accession # Q9UJ71
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human Langerin/CD207 (Catalog # 2088-LN)
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Langerin/CD207 in Human Immature Dentritic Cells by Flow Cytometry. Human immature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+ cells were treated with 20 ng/mL Recombinant Human IL‑4 (Catalog # 204-IL) and 50 ng/mL Recombinant Human GM‑CSF (Catalog # 215-GM) for 6‑7 days and then stained with Mouse Anti-Human Langerin/CD207 Monoclonal Antibody (Catalog # MAB2088, filled histogram) or isotype control (Catalog # MAB002, open histogram), followed by Goat F(ab)2 Anti-Mouse IgG (H+L) Allophycocyanin (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Langerin/CD207

Langerin (also known as CD207) is a type II transmembrane glycoprotein which is member K of the C-type lectin domain family 4 (1). Langerin is used as a marker for Langerhans cells (LCs) which represent the immature dendritic cells in the epidermis (1, 2). LCs uniquely contain “tennis racket”-shaped endosomal recycling compartment subdomains with pentalamellar membranes termed Birbeck granules (1‑3). Langerin is necessary and sufficient for Birbeck granule formation (1). The 328 amino acid (aa) human langerin sequence contains a 43 aa cytoplasmic domain, a 21 aa transmembrane domain and a 264 aa extracellular domain (ECD) that contains a coiled-coil domain and a single C-type lectin domain. Trimerization greatly increases the lectin binding affinity (4). Langerin internalizes endogenous proteins such as type I procollagen. Internalization by LC is thought to lead to suppression of self reactions (4‑6). Langerin also mediates endocytosis of non‑peptide antigens containing mannose, N-acetyl glucosamine and fucose that are expressed by mycobacteria and fungae (4, 7). Some antigens, such as the M. leprae glycolipid arabinomycolate, are ultimately presented by human LC CD1a in cutaneous-draining lymph nodes (8). Langerin performs a barrier-like function to HIV-1 transmission due to its internalization of virus particles for destruction (9). A rare human polymorphism within the lectin domain, W264R, abolishes both carbohydrate recognition and Birbeck granule formation (10, 11). Genetic deletion of mouse langerin was not shown to have functional consequence other than abolishing Birbeck granule formation (12). Human langerin shares 68%, 62%, 71% aa identity with mouse, rat and bovine langerin ECD, respectively.

  • References:
    1. Valladeau, J. et al. (2000) Immunity 12:71. 
    2. Valladeau, J. et al. (2003) Immunol. Res. 28:93. 
    3. McDermott, R. et al. (2002) Mol. Biol. Cell 13:317. 
    4. Stambach, N. S. and M. E. Taylor (2003) Glycobiology 13:401. 
    5. Tada, Y. et al. (2006) J. Invest. Dermatol. 126:1549. 
    6. Ritter, U. and A. Osterloh (2007) Med. Microbiol. Immunol. 196:51. 
    7. Takahara, K. et al. (2003) Int. Immunol. 16:819.
    8. Hunger, R. E. et al. (2004) J. Clin. Invest. 113:701.
    9. De Witte, L. et al. (2007) Nat. Med. 13:367.
    10. Verdijk, P. et al. (2005) J. Invest. Dermatol. 124:714.
    11. Ward, E. M. et al. (2006) J. Biol. Chem. 281:15450.
    12. Kissenpfennig, A. et al. (2005) Mol. Cell. Biol. 25:88.
  • Entrez Gene IDs:
    50489 (Human); 246278 (Mouse)
  • Alternate Names:
    CD207 antigen; CD207 antigen, langerin; CD207 molecule, langerin; CD207; CLEC4KLangerhans cell specific c-type lectin; C-type lectin domain family 4 member K; C-type lectin domain family 4, member K; Langerin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Notch is active in Langerhans cell histiocytosis and confers pathognomonic features on dendritic cells.
    Authors: Hutter, Caroline, Kauer, Max, Simonitsch-Klupp, Ingrid, Jug, Gunhild, Schwentner, Raphaela, Leitner, Judith, Bock, Peter, Steinberger, Peter, Bauer, Wolfgang, Carlesso, Nadia, Minkov, Milen, Gadner, Helmut, Stingl, Georg, Kovar, Heinrich, Kriehuber, Ernst
    Blood, 2012;120(26):5199-208.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  2. HIV-1 replication in Langerhans and interstitial dendritic cells is inhibited by neutralizing and Fc-mediated inhibitory antibodies.
    Authors: Peressin M, Holl V, Schmidt S, Decoville T, Mirisky D, Lederle A, Delaporte M, Xu K, Aubertin AM, Moog C
    J. Virol., 2011;85(2):1077-85.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell-derived lin-CD34+CD43+CD45+ progenitors.
    Authors: Choi KD, Vodyanik MA, Slukvin II
    J. Clin. Invest., 2009;119(9):2818-29.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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