Human LAP (TGF-beta 1) Quantikine ELISA Kit

  
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL), Platelet-poor Heparin Plasma (10 uL), Urine (50 uL)
  • Sensitivity
    3.4 pg/mL
  • Assay Range
    23.4 - 1,500 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine)
  • Specificity
    Natural and recombinant human LAP (TGF-beta 1) and Latent TGF-beta 1).
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC110, Quantikine Immunoassay Control Set 788 Human LAP (TGF-b1) - Please Inquire
Ancillary Reagent Kit Available
Product Summary
The Quantikine Human LAP (TGF-beta 1) Immunoassay is a 4.5 hour solid phase ELISA designed to measure LAP (TGF-beta 1) in acid activated cell culture supernates, serum, plasma, and urine. It contains recombinant human LAP (TGF-beta 1) expressed by CHO cells and has been shown to quantitate the recombinant factor accurately. Results obtained using natural LAP (TGF-beta 1) showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit will provide accurate quantitation for both recombinant and natural human LAP (TGF-beta 1).

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean2184279922164601126
Standard Deviation6.9217.742.17.6821.786.9
CV%3.24.14.23.64.77.7

Recovery

The recovery of LAP (TGF-beta 1) spiked to levels throughout the range of the assay followed by activation was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 98 90-105
Cell Lysates (n=4) 97 90-103
Urine (n=4) 95 87-101
Linearity
To assess the linearity of the assay, activated samples containing and/or spiked with high concentrations of LAP (TGF-beta 1) were diluted with Calibrator Diluent and then assayed.
Human LAP (TGF-beta 1) Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: LAP (TGF-beta 1)

Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. Targeted deletions of these genes in mice show that each TGF-beta isoform has some non-redundant functions: TGF-beta 1 is involved in hematopoiesis and endothelial differentiation; TGF-beta 2 affects development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems; and TGF-beta 3 influences palatogenesis and pulmonary development. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.

TGF-beta ligands are initially synthesized as precursor proteins that undergo proteolytic cleavage. The mature segments form active ligand dimers via a disulfide-rich core consisting of the characteristic 'cysteine knot'. TGF-beta signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II serine/threonine kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a type I serine/threonine kinase receptor, either ALK-1 or TGF-beta RI (also called ALK-5). The activated type I receptor phosphorylates and activates Smad proteins that regulate transcription. Use of other signaling pathways that are Smad-independent allows for distinct actions observed in response to TGF-beta in different contexts.

    • Long Name
      Latency-associated Peptide
    • Entrez Gene IDs
      7040 (Human);
    • Alternate Names
      LAP (TGFbeta 1); LAP (TGF-beta 1);
    Related Research Areas
    DuoSet Ancillary Reagent Kits
    Description Application Cat# Citations Images  

    Sample Activation Kit 1

    ELISA DY010
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 788 Human LAP (TGF-b1)

    Ctrl QC110
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....

    Cell Lysis Buffer 1 (1 x 21 mL)

    ELISA 890713 1
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....

    Have you used Human LAP (TGF-beta 1) Quantikine ELISA Kit?

    Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Order Details

    Contact Information

    R&D Systems Guarantee

    Feedback

    Customer Information