Human LAP TGF-beta 1 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM2462

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Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)

Human LAP TGF-beta 1 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Recognizes human LAP (TGF-beta 1) and human latent TGF-beta 1 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 27240
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1)
Leu30-Arg278 (Cys33Ser)
Accession # P01137
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample

Human LAP TGF-beta 1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Human LAP (TGF-beta 1) Antibody (Catalog # MAB2461)

Standard: Recombinant Human LAP (TGF-beta 1) Protein (Catalog # 246-LP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAP (TGF-beta 1)

The TGF-beta family includes several related proteins (70 - 80% sequence homology) from mammalian, avain or Xenopus systems that are now designated TGF-beta 1, TGF-beta 2, TGF-beta 1.2, TGF-beta 3, TGF-beta 4 and TGF-beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as latent TGF-beta, that consists of TGF-beta associated non-covalently with LAP. These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF-beta family members are naturally associated with their own distinct LAPs.

Long Name
Latency-associated Peptide
Entrez Gene IDs
7040 (Human)
Alternate Names
CED; DPD1; LAP (TGFbeta 1); LAP (TGF-beta 1); LAP; TGFB; TGFB1; TGFbeta; transforming growth factor beta 1

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Citations for Human LAP TGF-beta 1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. De novo design of highly selective miniprotein inhibitors of integrins ?v?6 and ?v?8
    Authors: Roy, A;Shi, L;Chang, A;Dong, X;Fernandez, A;Kraft, JC;Li, J;Le, VQ;Winegar, RV;Cherf, GM;Slocum, D;Poulson, PD;Casper, GE;Vallecillo-Zúniga, ML;Valdoz, JC;Miranda, MC;Bai, H;Kipnis, Y;Olshefsky, A;Priya, T;Carter, L;Ravichandran, R;Chow, CM;Johnson, MR;Cheng, S;Smith, M;Overed-Sayer, C;Finch, DK;Lowe, D;Bera, AK;Matute-Bello, G;Birkland, TP;DiMaio, F;Raghu, G;Cochran, JR;Stewart, LJ;Campbell, MG;Van Ry, PM;Springer, T;Baker, D;
    Nature communications
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. L(59) TGF-beta LAP degradation products serve as a promising blood biomarker for liver fibrogenesis in mice
    Authors: Mitsuko Hara, Ikuyo Inoue, Yuta Yamazaki, Akiko Kirita, Tomokazu Matsuura, Scott L. Friedman et al.
    Fibrogenesis & Tissue Repair
  3. Systemic and local characterization of regulatory T cells in a chronic fungal infection in humans.
    Authors: Cavassani KA, Campanelli AP, Moreira AP, Vancim JO, Vitali LH, Mamede RC, Martinez R, Silva JS
    J. Immunol., 2006-11-01;177(9):5811-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
    Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2004-09-16;105(2):750-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Flow Cytometry
  5. TGF-beta 1 plays an important role in the mechanism of CD4+CD25+ regulatory T cell activity in both humans and mice.
    Authors: Kitani A, Fuss I, Pedersen A, Nawata H
    J. Immunol., 2004-01-15;172(2):834-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human LAP TGF-beta 1 Biotinylated Antibody

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Human LAP TGF-beta 1 Biotinylated Antibody
By Anonymous on 03/29/2019
Application: ELISA Sample Tested: Recombinant protein Species: Human and Mouse

I tested the antibody using direct ELISA to see if it cross reacted with mouse latent TGF beta or mature mouse TGF beta 1 recombinant proteins.