Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

ELISA Capture (Matched Antibody Pair)

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 9005
View all LAP (TGF-beta 1) Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Latent TGF‑ beta 1
Leu30-Ser390
Accession # P01137

Specificity

Detects human LAP (TGF-beta 1) and Latent TGF-beta 1 in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human LAP (TGF-beta 1) Antibody

Human LAP TGF-beta 1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human LAP (TGF-beta 1) Biotinylated Antibody (Catalog # BAM2462)
  • Standard: Recombinant Human LAP (TGF-beta 1) Protein (Catalog # 246-LP)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LAP (TGF-beta 1)

The TGF-beta family includes several related proteins (70 - 80% sequence homology) from mammalian, avian or Xenopus systems that are now designated TGF-beta 1, TGF-beta 2, TGF-beta 1.2, TGF-beta 3, TGF-beta 4, and TGF-beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as Latent TGF-beta, that consists of TGF-beta associated non-covalently with a Latency-associated peptide (LAP). These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF-beta family members are naturally associated with their own distinct LAPs.

Long Name

Latency-associated Peptide

Alternate Names

LAP (TGFbeta 1)

Entrez Gene IDs

7040 (Human)

Gene Symbol

TGFB1

UniProt

P01137 (Human)

Additional LAP (TGF-beta 1) Products

Product Documents for Human LAP (TGF-beta 1) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human LAP (TGF-beta 1) Antibody

For research use only

Citations for Human LAP (TGF-beta 1) Antibody

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