Human LDLR Quantikine ELISA Kit

R&D Systems | Catalog # DLDLR0

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), Heparin Plasma (10 µL), Urine (50 µL)

Sensitivity

10.3 pg/mL

Assay Range

62.5-4000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, Heparin Plasma, Urine)
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Product Summary for Human LDLR Quantikine ELISA Kit

The Quantikine Human LDL R Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human LDL R in cell culture supernates, cell lysates, serum, heparin plasma, and urine. It contains NS0-expressed recombinant human LDL R and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human LDL R showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human LDL R.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human LDL R. This assay also recognizes recombinant human LDL R/PCSK9 complex.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Cell Lysates, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 260 787 1598 287 823 1602
Standard Deviation 5.68 17.5 32.4 15.6 40.0 74.4
CV% 2.2 2.2 2.0 5.4 4.9 4.6

Recovery for Human LDLR Quantikine ELISA Kit

The recovery of human LDL R spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 92-106
Urine (n=4) 94 91-99

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human LDL R were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.

Human LDL R ELISA Linearity

Scientific Data Images for Human LDLR Quantikine ELISA Kit

Human LDL R ELISA Standard Curve

Human LDL R ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LDLR

LDLR (Low Density Lipoprotein Receptor) is a widely expressed cell surface scavenger receptor. LDLR binds ApoB and ApoE, the apolipoproteins of low- and very low-density lipoproteins (LDL and VLDL), respectively. Hepatocyte LDLR is responsible for endocytosis and clearing of most plasma LDL cholesterol. At the low pH of the endocytic vesicle, it dissociates, allowing degradation of LDL and recycling of LDLR to the cell surface. Lack of LDLR expression or function causes familial hypercholesterolemia (FH). The protease PCSK9 can also cause increased plasma cholesterol by promoting LDLR degradation rather than recycling to the cell surface.

Long Name

Low Density Lipoprotein Receptor

Alternate Names

LDL R

Entrez Gene IDs

3949 (Human); 16835 (Mouse); 300438 (Rat); 396801 (Porcine); 102127361 (Cynomolgus Monkey)

Gene Symbol

LDLR

Additional LDLR Products

Product Documents for Human LDLR Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human LDLR Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Related Research Areas

Obesity

Citations for Human LDLR Quantikine ELISA Kit

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    Sample Tested: Human serum and Serum
    Verified Customer | Posted 10/23/2023
    Human LDLR Quantikine ELISA Kit DLDLR0

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Protocols

View specific protocols for Human LDLR Quantikine ELISA Kit (DLDLR0):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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