Human Leucyl-cystinyl Aminopeptidase/LNPEP Alexa Fluor® 532-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Leucyl-cystinyl Aminopeptidase/LNPEP

LNPEP (also known as P-LAP, CAP, IRAP, and Oxytocinase) is a 165-175 kDa member of the oxytocinase subfamily, M1 family of zinc metallopeptidases. It is widely expressed after cell stimulation and is found on endothelial cells, trophoblasts, skeletal muscle cells, neurons, adipocytes, and renal distal tubule epithelium. LNPEP cleaves vasopressin and oxytocin and serves as a receptor for AngIV. Human LNPEP is a 1025 amino acid (aa) type II transmembrane glycoprotein (SwissProt #:Q9UIQ6). It contains a 110 aa cytoplasmic region (aa 1-110) plus an 894 aa extracellular domain (aa 132-1025) that contains a peptidase M1 region (aa 168‑552). There are multiple potential splice variants. There are two alternative start sites at Met15 and Met20, and the cytoplasmic tail undergoes phosphorylation at Tyr70 and Ser80. LNPEP is reportedly forms homodimers. Proteolytic cleavage between Phe154Ala155 generates a 150-155 kDa soluble form. Over aa 133-552, human LNPEP shares 89% aa sequence identity with mouse LNPEP.

Product Datasheets

Related Research Areas

• Metalloproteases and Regulators
• Search Bioprocessing Enzymes
• Vasoactive Peptide Hormones and Regulators