Human Leucyl-cystinyl Aminopeptidase/LNPEP Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Leucyl-cystinyl Aminopeptidase/LNPEP in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) ENPEP, rhLRAP, and rhARTS1 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Leucyl-cystinyl Aminopeptidase/LNPEP
    Arg133-Gly552
    Accession # Q9UIQ6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with recombinant human Leucyl-cystinyl Aminopeptidase/LNPEP
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Leucyl-cystinyl Aminopeptidase/LNPEP by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human Leucyl-cystinyl Aminopeptidase/LNPEP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6386) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Leucyl-cystinyl Aminopeptidase/LNPEP at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Leucyl-cystinyl Aminopeptidase/LNPEP
LNPEP (also known as P-LAP, CAP, IRAP, and Oxytocinase) is a 165-175 kDa member of the oxytocinase subfamily, M1 family of zinc metallopeptidases. It is widely expressed after cell stimulation and is found on endothelial cells, trophoblasts, skeletal muscle cells, neurons, adipocytes, and renal distal tubule epithelium. LNPEP cleaves vasopressin and oxytocin and serves as a receptor for AngIV. Human LNPEP is a 1025 amino acid (aa) type II transmembrane glycoprotein (SwissProt #:Q9UIQ6). It contains a 110 aa cytoplasmic region (aa 1-110) plus an 894 aa extracellular domain (aa 132-1025) that contains a peptidase M1 region (aa 168‑552). There are multiple potential splice variants. There are two alternative start sites at Met15 and Met20, and the cytoplasmic tail undergoes phosphorylation at Tyr70 and Ser80. LNPEP is reportedly forms homodimers. Proteolytic cleavage between Phe154Ala155 generates a 150-155 kDa soluble form. Over aa 133-552, human LNPEP shares 89% aa sequence identity with mouse LNPEP.
  • Entrez Gene IDs:
    4012 (Human); 240028 (Mouse); 171105 (Rat)
  • Alternate Names:
    angiotensin IV receptor; AT (4) receptor; CAP; Cystinyl aminopeptidase; EC 3.4.11; EC 3.4.11.3; Insulin-regulated membrane aminopeptidase; Insulin-responsive aminopeptidase; IRAP; leucyl/cystinyl aminopeptidase; Leucylcystinyl Aminopeptidase; Leucyl-cystinyl Aminopeptidase; LNPEP; Otase; Oxytocinase; Placental leucine aminopeptidase; PLAP; P-LAP; P-LAPinsulin-regulated aminopeptidase; Vasopressinase
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