Human Leucyl-cystinyl Aminopeptidase/LNPEP Antibody

Catalog # Availability Size / Price Qty
AF6386
AF6386-SP
Human Leucyl-cystinyl Aminopeptidase/LNPEP Antibody in Western Blot
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Human Leucyl-cystinyl Aminopeptidase/LNPEP Antibody Summary

Species Reactivity
Human
Specificity
Detects human Leucyl-cystinyl Aminopeptidase/LNPEP in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) ENPEP, rhLRAP, and rhARTS1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Leucyl-cystinyl Aminopeptidase/LNPEP
Arg133-Gly552
Accession # Q9UIQ6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with recombinant human Leucyl-cystinyl Aminopeptidase/LNPEP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Leucyl-cystinyl Aminopeptidase/LNPEP by Western Blot. View Larger

Detection of Human Leucyl-cystinyl Aminopeptidase/LNPEP by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human Leucyl-cystinyl Aminopeptidase/LNPEP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6386) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Leucyl-cystinyl Aminopeptidase/LNPEP at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Leucyl-cystinyl Aminopeptidase/LNPEP

LNPEP (also known as P-LAP, CAP, IRAP, and Oxytocinase) is a 165-175 kDa member of the oxytocinase subfamily, M1 family of zinc metallopeptidases. It is widely expressed after cell stimulation and is found on endothelial cells, trophoblasts, skeletal muscle cells, neurons, adipocytes, and renal distal tubule epithelium. LNPEP cleaves vasopressin and oxytocin and serves as a receptor for AngIV. Human LNPEP is a 1025 amino acid (aa) type II transmembrane glycoprotein (SwissProt #:Q9UIQ6). It contains a 110 aa cytoplasmic region (aa 1-110) plus an 894 aa extracellular domain (aa 132-1025) that contains a peptidase M1 region (aa 168‑552). There are multiple potential splice variants. There are two alternative start sites at Met15 and Met20, and the cytoplasmic tail undergoes phosphorylation at Tyr70 and Ser80. LNPEP is reportedly forms homodimers. Proteolytic cleavage between Phe154Ala155 generates a 150-155 kDa soluble form. Over aa 133-552, human LNPEP shares 89% aa sequence identity with mouse LNPEP.

Entrez Gene IDs
4012 (Human); 240028 (Mouse); 171105 (Rat)
Alternate Names
angiotensin IV receptor; AT (4) receptor; CAP; Cystinyl aminopeptidase; EC 3.4.11; EC 3.4.11.3; Insulin-regulated membrane aminopeptidase; Insulin-responsive aminopeptidase; IRAP; leucyl/cystinyl aminopeptidase; Leucylcystinyl Aminopeptidase; Leucyl-cystinyl Aminopeptidase; LNPEP; Otase; Oxytocinase; Placental leucine aminopeptidase; PLAP; P-LAP; P-LAPinsulin-regulated aminopeptidase; Vasopressinase

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