Human Leucyl-cystinyl Aminopeptidase/LNPEP Alexa Fluor™ Plus 555‑conjugated Antibody

R&D Systems | Catalog # AF6386AFP555

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)

Antibody Source

Polyclonal Sheep IgG
View all Leucyl-cystinyl Aminopeptidase/LNPEP Primary Antibodies »

Product Specifications

Specificity

Detects human Leucyl-cystinyl Aminopeptidase/LNPEP in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) ENPEP, rhLRAP, and rhARTS1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications

Application
Recommended Usage

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Leucyl-cystinyl Aminopeptidase/LNPEP

LNPEP (also known as P-LAP, CAP, IRAP, and Oxytocinase) is a 165-175 kDa member of the oxytocinase subfamily, M1 family of zinc metallopeptidases. It is widely expressed after cell stimulation and is found on endothelial cells, trophoblasts, skeletal muscle cells, neurons, adipocytes, and renal distal tubule epithelium. LNPEP cleaves vasopressin and oxytocin and serves as a receptor for AngIV. Human LNPEP is a 1025 amino acid (aa) type II transmembrane glycoprotein (SwissProt #:Q9UIQ6). It contains a 110 aa cytoplasmic region (aa 1-110) plus an 894 aa extracellular domain (aa 132-1025) that contains a peptidase M1 region (aa 168‑552). There are multiple potential splice variants. There are two alternative start sites at Met15 and Met20, and the cytoplasmic tail undergoes phosphorylation at Tyr70 and Ser80. LNPEP is reportedly forms homodimers. Proteolytic cleavage between Phe154Ala155 generates a 150-155 kDa soluble form. Over aa 133-552, human LNPEP shares 89% aa sequence identity with mouse LNPEP.

Alternate Names

IRAP, Leucylcystinyl Aminopeptidase, LNPEP, Otase, Oxytocinase, P-LAP, Vasopressinase

Entrez Gene IDs

4012 (Human); 240028 (Mouse); 171105 (Rat)

Gene Symbol

LNPEP

UniProt

Q9UIQ6 (Human)

Additional Leucyl-cystinyl Aminopeptidase/LNPEP Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Metalloproteases and Regulators

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