Human LIGHT/TNFSF14 Alexa Fluor® 647-conjugated Antibody

Clone 115520 was used by HLDA to establish CD designation
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FAB664R-100UG
R&D Systems Antibodies
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Human LIGHT/TNFSF14 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human LIGHT/TNFSF14 in ELISAs. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) Fas Ligand, rhAPRIL, recombinant mouse TRANCE, rhTNF-alpha or rhTRAIL is observed.
Source
Monoclonal Mouse IgG1 Clone # 115520
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LIGHT/TNFSF14
Asp74-Val240
Accession # O43557
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Human CD3+ T cells treated with PMA and Ca2+ ionomycin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LIGHT/TNFSF14

Human LIGHT, also known as TNFSF14, is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT beta R) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma.

References
  1. Mauri, D.N. et al. (1998) Immunity 8:21.
  2. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
  3. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
  4. Yu, K-Y. et al. (1999) J. Biol. Chem. 274:13733.
Long Name
TNF Ligand Superfamily Member 14
Entrez Gene IDs
8740 (Human); 50930 (Mouse)
Alternate Names
CD258 antigen; CD258; delta transmembrane LIGHT; Herpes virus entry mediator ligand; Herpesvirus entry mediator ligand; HVEM-L; HVEMLherpesvirus entry mediator-ligand; ligand for herpesvirus entry mediator; LIGHT; LIGHTherpesvirus entry mediator A; LTg; TNFSF14; TR2; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand superfamily member 14; tumor necrosis factor receptor-like 2; tumor necrosis factor superfamily member LIGHT

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