Human LIGHT/TNFSF14 Antibody (115520)

R&D Systems | Catalog # MAB664

Clone 115520 was used by HLDA to establish CD designation
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA Capture (Matched Antibody Pair), Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 115520
View all LIGHT/TNFSF14 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LIGHT/TNFSF14
Asp74-Val240
Accession # O43557

Specificity

Detects human LIGHT/TNFSF14 in ELISAs. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) Fas Ligand, rhAPRIL, recombinant mouse TRANCE, rhTNF-alpha or rhTRAIL is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human LIGHT/TNFSF14 Antibody (115520)

Detection of LIGHT/TNFSF14 in PBMC treated with PMA and Calcium Ionomycin vs resting PBMC by Flow Cytometry

PBMC treated with 50ng/mL PMA and 500ng/mL Calcium Ionomycin for 24 hours (A) vs resting PBMC (B) were stained with Mouse Anti-Human LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB664) and Mouse Anti-Human CD8 alpha PE‑conjugated Monoclonal Antibody (Catalog # FAB1509P) isotype control antibody (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.
Human LIGHT / TNFSF14 ELISA Standard Curve

Human LIGHT / TNFSF14 ELISA Standard Curve

Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI) was serially diluted and captured by Mouse Anti-Human LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB664) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human LIGHT/TNFSF14 Antigen Affinity-purified Polyclonal Antibody (AF664) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998)

Applications for Human LIGHT/TNFSF14 Antibody (115520)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMC treated with PMA and Ca2+ ionomycin

Human LIGHT/TNFSF14 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human LIGHT/TNFSF14 Biotinylated Antibody (Catalog # BAF664)
  • Standard: Recombinant Human LIGHT/TNFSF14 Protein (Catalog # 664-LI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB664 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LIGHT/TNFSF14

Human LIGHT, also known as TNFSF14, is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT beta R) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma.

References

  1. Mauri, D.N. et al. (1998) Immunity 8:21.
  2. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
  3. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
  4. Yu, K-Y. et al. (1999) J. Biol. Chem. 274:13733.

Long Name

TNF Ligand Superfamily Member 14

Alternate Names

CD258, TNFSF14

Entrez Gene IDs

8740 (Human); 50930 (Mouse)

Gene Symbol

TNFSF14

UniProt

O43557

Additional LIGHT/TNFSF14 Products

Product Documents for Human LIGHT/TNFSF14 Antibody (115520)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LIGHT/TNFSF14 Antibody (115520)

For research use only

Citations for Human LIGHT/TNFSF14 Antibody (115520)

Customer Reviews for Human LIGHT/TNFSF14 Antibody (115520) (1)

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  • Human LIGHT/TNFSF14 Antibody
    Name: Sarah Garrett
    Application: Flow Cytometry
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 05/22/2018
    Cells were transfected with hLIGHT (IRES GFP) or a negative control. Cells were stained with MAB664 using a 1/500 dilution and binding was detected with an anti-mouse 594 secondary antibody.
    Human LIGHT/TNFSF14 Antibody (115520) MAB664

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