|Detection of LIGHT/TNFSF14 in Human CD3+ T cells by Flow Cytometry. Human CD3+ T cells treated with 1 ng/mL PMA and Calcium Ionomycin for 36 hours were stained with Mouse Anti-Human LIGHT/TNFSF14 APC‑conjugated Monoclonal Antibody (Catalog # FAB664A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). Cells were gate on CD4+ cells using Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P). View our protocol for Staining Membrane-associated Proteins.|
Human LIGHT, also known as TNFSF14, is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to Lymphotoxins, exhibits Inducible expression, and competes with HSV Glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the Lymphotoxin beta Receptor (LT beta R) and the decoy receptor, DcR3/TR6. LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma.
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