Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 292305
View all LILRB1/CD85j/ILT2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ILT2/CD85j
Gly24-His458
Accession # Q8NHL6

Specificity

Detects human ILT2/CD85j in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) ILT4, rhILT5, rhLIR6 or rhLIR8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human LILRB1/CD85j/ILT2 APC‑conjugated Antibody

Detection of LILRB1/CD85j/ILT2 antibody in Human Blood Monocytes antibody by Flow Cytometry.

Detection of LILRB1/CD85j/ILT2 in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Mouse Anti-Human LILRB1/CD85j/ILT2 APC-conjugated Monoclonal Antibody (Catalog # FAB20171A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human LILRB1/CD85j/ILT2 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood monocytes

Reviewed Applications

Read 2 reviews rated 5 using FAB20171A in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LILRB1/CD85j/ILT2

The immunoglobulin-like transcript (ILT) family of activating and inhibitory type immunoreceptors are expressed on many leukocyte subsets and function in the regulation of immune responses (1‑3). This family was also named leukocyte Ig-like receptors (LIR) and monocyte/macrophage Ig-like receptors (MIR). ILTs share significant homology with killer cell Ig-like receptors (KIR). The ILT genes are located on human chromosome 19q13.4 in the leukocyte receptor complex, which also include the genes encoding KIRs (4). With the exception of ILT-6, which is a soluble molecule, all ILT family members are type I transmembrane proteins having two or four extracellular Ig-like domains (2, 3). One subset of the ILT receptors (referred to as subfamily B of the LIRs) has long cytoplasmic tails containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events by recruiting SH2-containing protein tyrosine phosphatase-1. Another subset of the ILT receptors (referred to as subfamily A of the LIRs) contains activating receptors with short cytoplasmic regions that lack signal transduction motifs. These receptors contain a basic arginine residue within their transmembrane domains, which allows association with Fc R gamma, an immunoreceptor tyrosine-based activation motif (ITAM)-bearing signal adapter protein (1‑3). ILT2, also known as LIR1, MIR7, and CD85j, is expressed on most monocytes, dendritic cells, and mature B cells (1‑3). It is also expressed on small percentages of T cells and NK cells. ILT2 has four extracellular Ig-like domains and three cytoplasmic ITIMs. It functions as an inhibitory receptor that prevents cellular activation. ILT2 has been shown to bind classical (HLA-A and -B) and nonclassical (HLA-G1, -E and -F) MHC class I molecules (MHCI) (1‑3). ILT2 also binds with high affinity to an MHC class I homologue from human cytomegalovirus (3). Ligation of ILT2 by MHC class I may function to poise cellular activation thresholds and inhibit various leukocyte effector mechanisms that are regulated by MHC class I molecules on target cells.

References

  1. Allen, D. et al. (2000) Immunobiol. 202:34.
  2. Colonna, M. et al. (1999) J. Leukocyte Biol. 66:375.
  3. Borges, L. and D. Cosman (2000) Cytokine Growth Factor Rev. 11:209.
  4. Young, N. et al. (2001) Immunogenetics 53:270.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 1

Alternate Names

CD85j, ILT2, LIR1, MIR7

Entrez Gene IDs

10859 (Human)

Gene Symbol

LILRB1

UniProt

Q8NHL6

Additional LILRB1/CD85j/ILT2 Products

Product Documents for Human LILRB1/CD85j/ILT2 APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human LILRB1/CD85j/ILT2 APC‑conjugated Antibody

For research use only

Citations for Human LILRB1/CD85j/ILT2 APC‑conjugated Antibody

Customer Reviews for Human LILRB1/CD85j/ILT2 APC‑conjugated Antibody (2)

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  • Human LILRB1/CD85j/ILT2 APC-conjugated Antibody
    Name: Stephane Hua
    Application: Flow Cytometry
    Sample Tested: Dendritic cells
    Species: Human
    Verified Customer | Posted 07/05/2018
    Human LILRB1/CD85j/ILT2 APC‑conjugated Antibody FAB20171A
  • Human LILRB1/CD85j/ILT2 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human peripheral CD11c+ dendritic cells and Dendritic cells
    Species: Human
    Verified Customer | Posted 06/24/2018

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