Human LILRB3/CD85a/ILT5 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1806P-025
FAB1806P-100
Human LILRB3/CD85a/ILT5 PE-conjugated Antibody in Flow Cytometry
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Human LILRB3/CD85a/ILT5 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ILT5/CD85a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ILT1, rhILT2, rhILT3, rhILT4, rhILT6, rhILT7 or rhILT11 is observed.
Source
Monoclonal Mouse IgG2A Clone # 222821
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ILT5/CD85a
Gly24-Glu443
Accession # AAB68668
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of LILRB3/CD85a/ILT5 in Human PBMCs by Flow Cytometry. View Larger

Detection of LILRB3/CD85a/ILT5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on monocytes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human LILRB3/CD85a/ILT5 PE-conjugated Monoclonal Antibody (Catalog # FAB1806P) or (B) Mouse IgG2APhycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LILRB3/CD85a/ILT5

Immunoglobulin-like transcript 5 (ILT5), also known as leukocyte immunoglobulin-like receptor subfamily B (LILRB3), leukocyte immunoglobulin-like receptor 3 (LIR3) and CD85a, is an Ig-like receptor family member that is involved in immune regulation. ILT5 belongs to subfamily B whose members have cytoplasmic tails that contain immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1. The counterpart to subfamily B is subfamily A, whose members qualify as activating receptors that lack ITIMs, but signal through association with FcR gamma. ILTs share structural homology and chromosomal localization with the KIR family of receptors (1, 2). Mature ILT5 is a highly polymorphic 85‑95 kDa glycoprotein that consists of a 420 amino acid (aa) extracellular domain (ECD) with four Ig-like domains, a 21 aa transmembrane segment, and a 167 aa cytoplasmic domain with three ITIMs (3, 4). Alternate splicing generates an isoform with a 17 aa insertion in the juxtamembrane ECD. In mouse and rat, the LILRB3 gene encodes the PIR-B protein which has six Ig-like domains. Rodent PIR-B and human ILT5 share 55% aa sequence identity within common regions of their ECDs. Both PIR-B and ILT5 are receptors for S. aureus, and activation of these receptors by bacteria influences the innate immune response triggered by TLRs (4). ILT5 is expressed on the surface of peripheral monocytes, neutrophils, eosinophils, basophils, and mast cell progenitors but not on mature mast cells (5‑7). On basophils, cross-linking of ILT5 to the activating receptors ILT1/LIR7 or Fc epsilon RI inhibits the release of histamine, leukotriene C4, and IL-4 (6). On osteoclast precursors, ILT5 ligation inhibits RANKL or M-CSF induced differentiation (8). ILT5 is down‑regulated on macrophages in rheumatoid arthritis synovial tissue following successful treatment with DMARD anti-rheumatic drugs (9).

References
  1. Brown, D. et al. (2004) Tissue Antigens 64:215.
  2. Borges, L. and D. Cosman (2000) Cytokine Growth Factor Rev. 11:209.
  3. SwissProt Accession # O75022.
  4. Nakayama, M. et al. (2007) J. Immunol. 178:4250.
  5. Tedla, N. et al. (2003) Proc. Natl. Acad. Sci. 100:1174.
  6. Sloane, D.E. et al. (2004) Blood 104:2832.
  7. Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
  8. Mori, Y. et al. (2008) J. Immunol. 181:4742.
  9. Huynh, O.A. et al. (2007) Rheumatology 46:742.
Entrez Gene IDs
11025 (Human)
Alternate Names
CD85 antigen-like family member A; CD85a antigen; CD85a; HL9Immunoglobulin-like transcript 5; ILT5; ILT-5; ILT5CD85a; Leukocyte immunoglobulin-like receptor 3; leukocyte immunoglobulin-like receptor subfamily B member 3; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB3; LIR3; LIR3CD85A; LIR-3MGC138403; member 3; Monocyte inhibitory receptor HL9; PIRB

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Reviews for Human LILRB3/CD85a/ILT5 PE-conjugated Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human LILRB3/CD85a/ILT5 PE-conjugated Antibody
By Anonymous on 07/05/2018
Application: Flow Sample Tested: Dendritic cells Species: Human

Human LILRB3/CD85a/ILT5 PE-conjugated Antibody
By Anonymous on 06/24/2018
Application: Flow Sample Tested: Human peripheral CD11c+ dendritic cells,BDCA3 dendritic cells Species: Human

the antibody was used to analyzed the expression level of ILT5 in BDCA1,BDCA3 and pDCs.