Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 222821
View all LILRB3/CD85a/ILT5 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ILT5/CD85a
Gly24-Glu443
Accession # AAB68668

Specificity

Detects human ILT5/CD85a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ILT1, rhILT2, rhILT3, rhILT4, rhILT6, rhILT7 or rhILT11 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human LILRB3/CD85a/ILT5 PE‑conjugated Antibody

Detection of LILRB3/CD85a/ILT5 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of LILRB3/CD85a/ILT5 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) gated on monocytes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human LILRB3/CD85a/ILT5 PE-conjugated Monoclonal Antibody (Catalog # FAB1806P) or (B) Mouse IgG2APhycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human LILRB3/CD85a/ILT5 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample:   Human peripheral blood mononuclear cells (PBMCs) gated on monocytes

Reviewed Applications

Read 2 reviews rated 5 using FAB1806P in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LILRB3/CD85a/ILT5

Immunoglobulin-like transcript 5 (ILT5), also known as leukocyte immunoglobulin-like receptor subfamily B (LILRB3), leukocyte immunoglobulin-like receptor 3 (LIR3) and CD85a, is an Ig-like receptor family member that is involved in immune regulation. ILT5 belongs to subfamily B whose members have cytoplasmic tails that contain immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1. The counterpart to subfamily B is subfamily A, whose members qualify as activating receptors that lack ITIMs, but signal through association with FcR gamma. ILTs share structural homology and chromosomal localization with the KIR family of receptors (1, 2). Mature ILT5 is a highly polymorphic 85‑95 kDa glycoprotein that consists of a 420 amino acid (aa) extracellular domain (ECD) with four Ig-like domains, a 21 aa transmembrane segment, and a 167 aa cytoplasmic domain with three ITIMs (3, 4). Alternate splicing generates an isoform with a 17 aa insertion in the juxtamembrane ECD. In mouse and rat, the LILRB3 gene encodes the PIR-B protein which has six Ig-like domains. Rodent PIR-B and human ILT5 share 55% aa sequence identity within common regions of their ECDs. Both PIR-B and ILT5 are receptors for S. aureus, and activation of these receptors by bacteria influences the innate immune response triggered by TLRs (4). ILT5 is expressed on the surface of peripheral monocytes, neutrophils, eosinophils, basophils, and mast cell progenitors but not on mature mast cells (5‑7). On basophils, cross-linking of ILT5 to the activating receptors ILT1/LIR7 or Fc epsilon RI inhibits the release of histamine, leukotriene C4, and IL-4 (6). On osteoclast precursors, ILT5 ligation inhibits RANKL or M-CSF induced differentiation (8). ILT5 is down‑regulated on macrophages in rheumatoid arthritis synovial tissue following successful treatment with DMARD anti-rheumatic drugs (9).

References

  1. Brown, D. et al. (2004) Tissue Antigens 64:215.
  2. Borges, L. and D. Cosman (2000) Cytokine Growth Factor Rev. 11:209.
  3. SwissProt Accession # O75022.
  4. Nakayama, M. et al. (2007) J. Immunol. 178:4250.
  5. Tedla, N. et al. (2003) Proc. Natl. Acad. Sci. 100:1174.
  6. Sloane, D.E. et al. (2004) Blood 104:2832.
  7. Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
  8. Mori, Y. et al. (2008) J. Immunol. 181:4742.
  9. Huynh, O.A. et al. (2007) Rheumatology 46:742.

Alternate Names

CD85a, ILT5, LIR3

Entrez Gene IDs

11025 (Human)

Gene Symbol

LILRB3

UniProt

AAB68668

Additional LILRB3/CD85a/ILT5 Products

Product Documents for Human LILRB3/CD85a/ILT5 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LILRB3/CD85a/ILT5 PE‑conjugated Antibody

For research use only

Customer Reviews for Human LILRB3/CD85a/ILT5 PE‑conjugated Antibody (2)

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Showing  1 - 2 of 2 reviews Showing All
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  • Human LILRB3/CD85a/ILT5 PE-conjugated Antibody
    Name: Stephane Hua
    Application: Flow Cytometry
    Sample Tested: Dendritic cells
    Species: Human
    Verified Customer | Posted 07/05/2018
    Human LILRB3/CD85a/ILT5 PE‑conjugated Antibody FAB1806P
  • Human LILRB3/CD85a/ILT5 PE-conjugated Antibody
    Name: ming sun
    Application: Flow Cytometry
    Sample Tested: Human peripheral CD11c+ dendritic cells and BDCA3 dendritic cells
    Species: Human
    Verified Customer | Posted 06/24/2018
    the antibody was used to analyzed the expression level of ILT5 in BDCA1,BDCA3 and pDCs.

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