Human LILRB3/CD85a/ILT5 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human ILT5/CD85a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ILT1, rhILT2, rhILT3, rhILT4, rhILT6, rhILT7 or rhILT11 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 222821
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ILT5/CD85a
    Accession # AAB68668
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of LILRB3/CD85a/ILT5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on monocytes were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human LILRB3/CD85a/ILT5 PE‑conjugated Monoclonal Antibody (Catalog # FAB1806P) or (B) Mouse IgG2A Phycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LILRB3/CD85a/ILT5

Immunoglobulin-like transcript 5 (ILT5), also known as leukocyte immunoglobulin-like receptor subfamily B (LILRB3), leukocyte immunoglobulin-like receptor 3 (LIR3) and CD85a, is an Ig-like receptor family member that is involved in immune regulation. ILT5 belongs to subfamily B whose members have cytoplasmic tails that contain immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1. The counterpart to subfamily B is subfamily A, whose members qualify as activating receptors that lack ITIMs, but signal through association with FcR gamma. ILTs share structural homology and chromosomal localization with the KIR family of receptors (1, 2). Mature ILT5 is a highly polymorphic 85‑95 kDa glycoprotein that consists of a 420 amino acid (aa) extracellular domain (ECD) with four Ig-like domains, a 21 aa transmembrane segment, and a 167 aa cytoplasmic domain with three ITIMs (3, 4). Alternate splicing generates an isoform with a 17 aa insertion in the juxtamembrane ECD. In mouse and rat, the LILRB3 gene encodes the PIR-B protein which has six Ig-like domains. Rodent PIR-B and human ILT5 share 55% aa sequence identity within common regions of their ECDs. Both PIR-B and ILT5 are receptors for S. aureus, and activation of these receptors by bacteria influences the innate immune response triggered by TLRs (4). ILT5 is expressed on the surface of peripheral monocytes, neutrophils, eosinophils, basophils, and mast cell progenitors but not on mature mast cells (5‑7). On basophils, cross-linking of ILT5 to the activating receptors ILT1/LIR7 or Fc epsilon RI inhibits the release of histamine, leukotriene C4, and IL-4 (6). On osteoclast precursors, ILT5 ligation inhibits RANKL or M-CSF induced differentiation (8). ILT5 is down‑regulated on macrophages in rheumatoid arthritis synovial tissue following successful treatment with DMARD anti-rheumatic drugs (9).

  • References:
    1. Brown, D. et al. (2004) Tissue Antigens 64:215.
    2. Borges, L. and D. Cosman (2000) Cytokine Growth Factor Rev. 11:209.
    3. SwissProt Accession # O75022.
    4. Nakayama, M. et al. (2007) J. Immunol. 178:4250.
    5. Tedla, N. et al. (2003) Proc. Natl. Acad. Sci. 100:1174.
    6. Sloane, D.E. et al. (2004) Blood 104:2832.
    7. Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
    8. Mori, Y. et al. (2008) J. Immunol. 181:4742.
    9. Huynh, O.A. et al. (2007) Rheumatology 46:742.
  • Entrez Gene IDs:
    11025 (Human)
  • Alternate Names:
    CD85 antigen-like family member A; CD85a antigen; CD85a; HL9Immunoglobulin-like transcript 5; ILT5; ILT-5; ILT5CD85a; Leukocyte immunoglobulin-like receptor 3; leukocyte immunoglobulin-like receptor subfamily B member 3; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LIR3; LIR3CD85A; LIR-3MGC138403; member 3; Monocyte inhibitory receptor HL9; PIRB
Related Research Areas
Isotype Controls
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Mouse IgG2A PE-conjugated Antibody

Ctrl IC003P 10  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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