Human LILRB3/CD85a/ILT5 Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human LILRB3/CD85a/ILT5 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ILT1, rhILT2, rhILT3, rhILT4, rhILT6, rhILT7 or rhILT11 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 222821
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human LILRB3/CD85a/ILT5
    Gly24-Glu443
    Accession # AAB68668
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human LILRB3/CD85a/ILT5 Fc Chimera (Catalog # 1806-T5)
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of LILRB3/CD85a/ILT5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human LILRB3/CD85a/ILT5 Monoclonal Antibody (Catalog # MAB1806) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LILRB3/CD85a/ILT5

Immunoglobulin-Like Transcript 5 (ILT5), also known as Leukocyte Immunoglobulin-Like Receptor subfamily B (LILRB3), leukocyte immunoglobulin-Like Receptor 3 (LIR3) and CD85a, is an Ig-like receptor family member that is involved in immune regulation. ILT5 belongs to subfamily B whose members have cytoplasmic tails that contain Immunoreceptor Tyrosine-based Inhibitory Motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1. The counterpart to subfamily B is subfamily A, whose members qualify as activating receptors that lack ITIMs, but signal through association with FcR gamma. ILTs share structural homology and chromosomal localization with the KIR family of receptors (1, 2). Mature ILT5 is a highly polymorphic 85‑95 kDa glycoprotein that consists of a 420 amino acid (aa) extracellular domain (ECD) with four Ig-like domains, a 21 aa transmembrane segment, and a 167 aa cytoplasmic domain with three ITIMs (3, 4). Alternate splicing generates an isoform with a 17 aa insertion in the juxtamembrane ECD. In mouse and rat, the LILRB3 gene encodes the PIR-B protein which has six Ig-like domains. Rodent PIR-B and human ILT5 share 55% aa sequence identity within common regions of their ECDs. Both PIR-B and ILT5 are receptors for S. aureus, and activation of these receptors by bacteria influences the innate immune response triggered by TLRs (4). ILT5 is expressed on the surface of peripheral monocytes, neutrophils, eosinophils, basophils, and mast cell progenitors but not on mature mast cells (5‑7). On basophils, cross-linking of ILT5 to the activating receptors ILT1/LIR7 or Fc epsilon RI inhibits the release of Histamine, Leukotriene C4, and IL-4 (6). On osteoclast precursors, ILT5 ligation inhibits RANKL or M-CSF induced differentiation (8). ILT5 is down‑regulated on macrophages in rheumatoid arthritis synovial tissue following successful treatment with DMARD anti-rheumatic drugs (9).

  • References:
    1. Brown, D. et al. (2004) Tissue Antigens 64:215.
    2. Borges, L. and D. Cosman (2000) Cytokine Growth Factor Rev. 11:209.
    3. SwissProt Accession # O75022.
    4. Nakayama, M. et al. (2007) J. Immunol. 178:4250.
    5. Tedla, N. et al. (2003) Proc. Natl. Acad. Sci. 100:1174.
    6. Sloane, D.E. et al. (2004) Blood 104:2832.
    7. Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
    8. Mori, Y. et al. (2008) J. Immunol. 181:4742.
    9. Huynh, O.A. et al. (2007) Rheumatology 46:742.
  • Entrez Gene IDs:
    11025 (Human)
  • Alternate Names:
    CD85 antigen-like family member A; CD85a antigen; CD85a; HL9Immunoglobulin-like transcript 5; ILT5; ILT-5; ILT5CD85a; Leukocyte immunoglobulin-like receptor 3; leukocyte immunoglobulin-like receptor subfamily B member 3; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB3; LIR3; LIR3CD85A; LIR-3MGC138403; member 3; Monocyte inhibitory receptor HL9; PIRB
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Leukocyte immunoglobulin-like receptors maintain unique antigen-presenting properties of circulating myeloid dendritic cells in HIV-1-infected elite controllers.
    Authors: Huang J, Burke PS, Cung TD
    J. Virol., 2010;84(18):9463-71.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Cytokine induced expression of programmed death ligands in human neutrophils.
    Authors: Bankey PE, Banerjee S, Zucchiatti A, De M, Sleem RW, Lin CF, Miller-Graziano CL, De AK
    Immunol. Lett., 2010;129(2):100-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Allogeneic disparities in immunoglobulin-like transcript 5 induce potent antibody responses in hematopoietic stem cell transplant recipients.
    Authors: Pfistershammer K, Lawitschka A, Klauser C, Leitner J, Weigl R, Heemskerk MH, Pickl WF, Majdic O, Bohmig GA, Fischer GF, Greinix HT, Steinberger P
    Blood, 2009;114(11):2323-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Inhibitory immunoglobulin-like receptors LILRB and PIR-B negatively regulate osteoclast development.
    Authors: Mori Y, Tsuji S, Inui M, Sakamoto Y, Endo S, Ito Y, Fujimura S, Koga T, Nakamura A, Takayanagi H, Itoi E, Takai T
    J. Immunol., 2008;181(7):4742-51.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Cis binding between inhibitory receptors and MHC class I can regulate mast cell activation.
    Authors: Masuda A, Nakamura A, Maeda T, Sakamoto Y, Takai T
    J. Exp. Med., 2007;204(4):907-20.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Mouse IgG2A Antibody

Flow MAB0032  
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