Human MBP DuoSet ELISA

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Ancillary Products Available
Human MBP ELISA Standard Curve
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Product Details
Citations (7)
Supplemental Products

Human MBP DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Assay Range
234.4 - 15,000 pg/mL
Sufficient Materials
For five 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Myelin Basic Protein (MBP). The suggested diluent is suitable for the analysis of most cell culture supernate, serum, plasma, and cerebrospinal fluid samples. Diluents for complex matrices, such as serum, plasma, cerebrospinal fluid, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

Human MBP ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Assay Procedure


Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human MBP DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Chinese patients with hereditary spastic paraplegias (HSPs): a protocol for a hospital-based cohort study
    Authors: YS Qiu, YH Zeng, RY Yuan, ZX Ye, J Bi, XH Lin, YJ Chen, MW Wang, Y Liu, SB Yao, YK Chen, JY Jiang, Y Lin, X Lin, N Wang, Y Fu, WJ Chen
    BMJ Open, 2022;12(1):e054011.
    Species: Human
    Sample Types: Plasma
  2. Safety of Special Waveform of Transcranial Electrical Stimulation (TES): In Vivo Assessment
    Authors: M Adeel, CC Chen, BS Lin, HC Chen, JC Liou, YT Li, CW Peng
    International Journal of Molecular Sciences, 2022;23(12):.
    Species: Rat
    Sample Types: Tissue Homogenates
  3. Distribution of five clinically important neuroglial proteins in the human brain
    Authors: K Sjölin, K Kultima, A Larsson, E Freyhult, C Zjukovskaj, K Alkass, J Burman
    Molecular Brain, 2022;15(1):52.
    Species: Human
    Sample Types: Tissue Homogenates
  4. Biomarkers of demyelination and axonal damage are decreased after autologous hematopoietic stem cell transplantation for multiple sclerosis
    Authors: C Zjukovskaj, A Larsson, H Cherif, K Kultima, J Burman
    Multiple sclerosis and related disorders, 2022;68(0):104210.
    Species: Human
    Sample Types: CSF
  5. Relationship between cerebrospinal fluid biomarkers of inflammation and tissue damage in primary progressive multiple sclerosis
    Authors: J Talbot, H Højsgaard, M Mahler, S Buhelt, R Holm Hanse, H Lundell, T Vinther-Je, MNN Hellem, JE Nielsen, HR Siebner, MR von Essen, F Sellebjerg
    Multiple sclerosis and related disorders, 2022;68(0):104209.
    Species: Human
    Sample Types: CSF
  6. Carbon monoxide (CO) correlates with symptom severity, autoimmunity, and responses to probiotics treatment in a cohort of children with autism spectrum disorder (ASD): a post-hoc analysis of a randomized controlled trial
    Authors: HT Sherman, K Liu, K Kwong, ST Chan, AC Li, XJ Kong
    BMC Psychiatry, 2022;22(1):536.
    Species: Human
    Sample Types: Serum
  7. Dexmedetomidine Increases MMP-12 and MBP Concentrations after Coronary Artery Bypass Graft Surgery with Extracorporeal Circulation Anaesthesia without Impacting Cognitive Function: A Randomised Control Trial
    Authors: M Kowalczyk, A Panasiuk-K, A Stadnik, M Guz, M Cybulski, W Jeleniewic, A Stepulak, M Kwiatosz-M
    International Journal of Environmental Research and Public Health, 2022;19(24):.
    Species: Human
    Sample Types: Serum


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