Myelin Basic Protein (MBP) is the most abundant protein component of the myelin membrane in the central nervous system (CNS). Human MBP has 304 amino acids (aa) and shows 71% and 49% aa sequence identity with the mouse and rat orthologs, respectively. Alternative splicing of a single gene, and the optional posttranslational modification of different aa residues, produces a spectrum of isoforms that range in size from 14 kDa to 21.5 kDa. These isoforms exhibit different developmental and cellular expression patterns, and may have different specialized functions. MBP has a role in both the formation and stabilization of the myelin membrane in the CNS. It has also been suggested that MBP is involved in signaling pathways in neural cells and T cells. Research has shown that antibodies against MBP play a role in the development of multiple sclerosis (MS) for a subset of patients. Additionally, elevated levels of MBP in the cerebrospinal fluid is thought to be a sign of myelin breakdown and could serve as a biomarker for several neurodegenerative diseases including MS, traumatic brain injury, and stroke.
Key Product Details
Assay Type
Assay Range
Sample Type
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human MBP DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human MBP DuoSet ELISA
Product Specifications
Assay Format
Sample Volume Required
Detection Method
Conjugate
Specificity
Label
Kit Contents for Human MBP DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 3 (5 plates): (Catalog # DY009C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 3.
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)
Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)
Microplates: (Catalog # DY990), or equivalent
Plate Sealers: (Catalog # DY992), or equivalent
*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.
Preparation and Storage
Shipping
Stability & Storage
Background: MBP
Long Name
Alternate Names
Gene Symbol
Additional MBP Products
Product Documents for Human MBP DuoSet ELISA
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human MBP DuoSet ELISA
For research use only
Related Research Areas
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