Human MDL-1/CLEC5A APC-conjugated Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human MDL‑1/CLEC5A in Western blots. Does not cross-react with recombinant mouse MDL‑1.
  • Source
    Monoclonal Mouse IgG2B Clone # 283834
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human MDL‑1/CLEC5A
    Tyr26-Lys188
    Accession # Q9NY25
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of MDL‑1/CLEC5A in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with (A) MouseAnti-Human Siglec-3/CD33 PE-conjugated Monoclonal Antibody (Catalog # FAB1137P) and (B) Mouse Anti-Human MDL‑1/CLEC5A APC‑conjugated Monoclonal Antibody (Catalog # FAB2384A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: MDL-1/CLEC5A
MDL-1 is an approximately 40 kDa transmembrane glycoprotein belonging to the C-type lectin superfamily. MDL-1 is expressed on immature myeloid cells, monocytes, macrophages, dendritic cells, neutrophils, NK cells, and osteocytes. It contains a charged lysine in the transmembrane region that enables it to associate with DAP12 and deliver an activating signal. MDL-1 mediates inflammatory responses during autoimmune arthritis and upon binding to Dengue and Japanese encephalitis viruses. The extracellular domain (ECD) of MDL-1 contains a juxtamembrane stalk region and one C-type lectin domain. Within the ECD, human and mouse MDL-1 share 67% amino acid sequence identity.
  • Long Name:
    Myeloid DAP12-associating Lectin
  • Entrez Gene IDs:
    23601 (Human); 23845 (Mouse)
  • Alternate Names:
    CLEC5A; CLECSF5; CLECSF5carbohydrate-recognition domain) lectin, superfamilymember 5; C-type lectin domain family 5, member A; C-type lectin superfamily member 5; MDL1; MDL-1; MDL-1MGC138304; Myeloid DAP12-associating lectin 1; myeloid DAP12-associating lectin-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Phenotypic and functional consequences of different isolation protocols on skin mononuclear phagocytes
    Authors: RA Botting, KM Bertram, H Baharlou, KJ Sandgren, J Fletcher, JW Rhodes, H Rana, TM Plasto, XM Wang, JJ Lim, L Barnouti, MP Kohout, T Papadopoul, S Merten, N Olbourne, AL Cunningham, M Haniffa, AN Harman
    J. Leukoc. Biol, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human MDL-1/CLEC5A APC-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information