Human MDL-1/CLEC5A Antibody

Catalog # Availability Size / Price Qty
MAB2384
MAB2384-SP
Detection of MDL‑1/CLEC5A in Human Blood Granulocytes by Flow Cytometry.
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Product Details
Citations (3)
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Human MDL-1/CLEC5A Antibody Summary

Species Reactivity
Human
Specificity
Detects human MDL‑1/CLEC5A in Western blots. Does not cross-react with recombinant mouse MDL‑1.
Source
Monoclonal Mouse IgG2B Clone # 283834
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MDL‑1/CLEC5A
Tyr26-Lys188
Accession # Q9NY25
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MDL‑1/CLEC5A under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MDL-1/CLEC5A antibody in Human Blood Granulocytes antibody by Flow Cytometry. View Larger

Detection of MDL‑1/CLEC5A in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with (A) Mouse Anti-Human Siglec-3/CD33 APC-conjugated Monoclonal Antibody (Catalog # FAB1137A) and (B) Mouse Anti-Human MDL-1/CLEC5A Monoclonal Antibody (Catalog # MAB2384, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MDL-1/CLEC5A

MDL-1 is an approximately 40 kDa transmembrane glycoprotein belonging to the C-type lectin superfamily (CLEC5A). MDL-1 is expressed on immature myeloid cells, monocytes, macrophages, dendritic cells, neutrophils, NK cells, and osteocytes. It contains a charged lysine in the transmembrane region that enables it to associate with DAP12 and deliver an activating signal. MDL-1 mediates inflammatory responses during autoimmune arthritis and upon binding to Dengue and Japanese encephalitis viruses. The extracellular domain (ECD) of MDL-1 contains a juxtamembrane stalk region and one C-type lectin domain. Within the ECD, human and mouse MDL-1 share 67% amino acid sequence identity.

Long Name
Myeloid DAP12-associating Lectin
Entrez Gene IDs
23601 (Human); 23845 (Mouse)
Alternate Names
CLEC5A; CLECSF5; CLECSF5carbohydrate-recognition domain) lectin, superfamilymember 5; C-type lectin domain family 5, member A; C-type lectin superfamily member 5; MDL1; MDL-1; MDL-1MGC138304; Myeloid DAP12-associating lectin 1; myeloid DAP12-associating lectin-1

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Citations for Human MDL-1/CLEC5A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Cutting Edge: CLEC5A Mediates Macrophage Function and Chronic Obstructive Pulmonary Disease Pathologies
    Authors: BW Wortham, BL Eppert, JL Flury, SM Garcia, WR Donica, A Osterburg, B Joyce-Shai, DJ Cua, MT Borchers
    J. Immunol., 2016-02-29;196(8):3227-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Atypical Activin A and IL-10 Production Impairs Human CD16+ Monocyte Differentiation into Anti-Inflammatory Macrophages.
    Authors: Gonzalez-Dominguez E, Dominguez-Soto A, Nieto C, Flores-Sevilla J, Pacheco-Blanco M, Campos-Pena V, Meraz-Rios M, Vega M, Corbi A, Sanchez-Torres C
    J Immunol, 2016-01-04;196(3):1327-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Comparison of gene expression profiles between human and mouse monocyte subsets.
    Authors: Ingersoll MA, Spanbroek R, Lottaz C
    Blood, 2009-11-12;115(3):e10-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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