Human MDL-1/CLEC5A PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2384P
Detection of MDL‑1/CLEC5A in Human Blood Granulocytes by Flow Cytometry.
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Human MDL-1/CLEC5A PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human MDL‑1/CLEC5A in Western blots. Does not cross-react with recombinant mouse MDL‑1.
Source
Monoclonal Mouse IgG2B Clone # 283834
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MDL‑1/CLEC5A
Tyr26-Lys188
Accession # Q9NY25
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MDL-1/CLEC5A antibody in Human Blood Granulocytes antibody by Flow Cytometry. View Larger

Detection of MDL‑1/CLEC5A in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with (A) Mouse Anti-Human Siglec-3/CD33 APC-conjugated Monoclonal Antibody (Catalog # FAB1137A) and (B) Mouse Anti-Human MDL-1/CLEC5A PE-conjugated Monoclonal Antibody (Catalog # FAB2384P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: MDL-1/CLEC5A

MDL-1 is an approximately 40 kDa transmembrane glycoprotein belonging to the C-type lectin superfamily. MDL-1 is expressed on immature myeloid cells, monocytes, macrophages, dendritic cells, neutrophils, NK cells, and osteocytes. It contains a charged lysine in the transmembrane region that enables it to associate with DAP12 and deliver an activating signal. MDL-1 mediates inflammatory responses during autoimmune arthritis and upon binding to Dengue and Japanese encephalitis viruses. The extracellular domain (ECD) of MDL-1 contains a juxtamembrane stalk region and one C-type lectin domain. Within the ECD, human and mouse MDL-1 share 67% amino acid sequence identity.

Long Name
Myeloid DAP12-associating Lectin
Entrez Gene IDs
23601 (Human); 23845 (Mouse)
Alternate Names
CLEC5A; CLECSF5; CLECSF5carbohydrate-recognition domain) lectin, superfamilymember 5; C-type lectin domain family 5, member A; C-type lectin superfamily member 5; MDL1; MDL-1; MDL-1MGC138304; Myeloid DAP12-associating lectin 1; myeloid DAP12-associating lectin-1

Product Datasheets

You must select a language.

x

Citations for Human MDL-1/CLEC5A PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Activation of the Innate Immune Checkpoint CLEC5A on Myeloid Cells in the Absence of Danger Signals Modulates Macrophages' Function but Does Not Trigger the Adaptive T Cell Immune Response
    Authors: MJ Tosiek, K Groesser, A Pekcec, M Zwirek, G Murugesan, E Borges
    Journal of Immunology Research, 2022-02-25;2022(0):9926305.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Elevated Expression of C-Type Lectin Domain Family 5-Member A (CLEC5A) and Its Relation to Inflammatory Parameters and Disease Course in Adult-Onset Still's Disease
    Authors: PK Chen, SL Hsieh, JL Lan, CC Lin, SH Chang, DY Chen
    J Immunol Res, 2020-04-23;2020(0):9473497.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human MDL-1/CLEC5A PE-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human MDL-1/CLEC5A PE-conjugated Antibody and earn rewards!

Have you used Human MDL-1/CLEC5A PE-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review